Identification of a conserved sequence in the non-coding regions of many human genes

Journal Article · Wed Jan 25 00:00:00 EST 1989 · Nucleic Acids Research; (UK)

DOI:https://doi.org/10.1093/nar/17.2.699· OSTI ID:5266058

Donehower, L A; Slagle, B L; Wilde, M; Darlington, G; Butel, J S ^[1]

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1. Baylor College of Medicine, Houston, TX (USA)

The authors have analyzed a sequence of approximately 70 base pairs (bp) that shows a high degree of similarity to sequences present in the non-coding regions of a number of human and other mammalian genes. The sequence was discovered in a fragment of human genomic DNA adjacent to an integrated hepatitis B virus genome in cells derived from human hepatocellular carcinoma tissue. When one of the viral flanking sequences was compared to nucleotide sequences in GenBank, more than thirty human genes were identified that contained a similar sequence in their non-coding regions. This element was highly conserved at the same position within the corresponding human and mouse genes for myoglobin and N-myc, indicating evolutionary conservation and possible functional importance. Preliminary DNase I footprinting data suggested that the element or its adjacent sequences may bind nuclear factors to generate specific DNase I hypersensitive sites. The size, structure, and evolutionary conservation of this sequence indicates that it is distinct from other types of short interspersed repetitive elements. It is possible that the element may have a cis-acting functional role in the genome.

• OSTI ID: 5266058
• Journal Information: Nucleic Acids Research; (UK), Vol. 17:2; ISSN 0305-1048
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
CODONS
DNA SEQUENCING
GENES
MOLECULAR STRUCTURE
BIOLOGICAL EVOLUTION
DNA-ASE
ELECTROPHORESIS
GENE REGULATION
MAN
PHOSPHORUS 32
VIRUSES
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
DAYS LIVING RADIOISOTOPES
ENZYMES
ESTERASES
HYDROLASES
ISOTOPES
LIGHT NUCLEI
MAMMALS
MICROORGANISMS
NUCLEI
ODD-ODD NUCLEI
PARASITES
PHOSPHODIESTERASES
PHOSPHORUS ISOTOPES
PRIMATES
RADIOISOTOPES
STRUCTURAL CHEMICAL ANALYSIS
VERTEBRATES
550200* - Biochemistry