Fluidic assembly for an ultra-high-speed chromosome flow sorter
A fluidic assembly for an ultra-high-speed chromosome flow sorter using a fluid drive system of high pressure in the range of 250 to 1000 psi for greater flow velocity, a nozzle with an orifice having a small ratio of length to diameter for laminar flow rates well above the critical Reynolds number for the high flow velocity, and means for vibrating the nozzle along its axis at high frequencies in a range of about 300 kHz to 800 kHz ae described. The orifice is provided with a sharp edge at its inlet, and a conical section at its outlet for a transition from a short cylindrical aperture of small length to diameter ratio to free space. Sample and sheath fluids in separte low pressure reservoirs are transferred into separate high pressure buffer reservoirs through valve means which first permit the fluids to be loaded into the buffer reservoirs under low pressure. Once loaded, the buffer reservoirs are subjected ato high pressure and valves are operated to permit the buffer reservoirs to be emptied through the nozzle under high pressure. A sensor and decision logic is positioned at the exit of the nozzle, and a charging pulse is applied to the jet when a particle reaches a position further downstream where the droplets are formed. In order to adjust the timing of charge pulses, the distance between the sensing station at the outlet of the nozzle and the droplet breakoff point is determined by stroboscopic illumination of the droplet breakoff region using a laser and a revolving lucite cylinder for breaking up the coherency of the laser, and a beam on/off modulator. The breakoff point in the region thus illuminated may then be viewed, using a television monitor.
- DOE Contract Number:
- W-7405-ENG-48
- Assignee:
- Dept. of Energy
- OSTI ID:
- 5246047
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
47 OTHER INSTRUMENTATION
CHROMOSOMES
SORTING
FLUIDIC CONTROL DEVICES
DESIGN
FLUID FLOW
FLUORESCENCE
MEASURING METHODS
PARTICLE SIZE
SEPARATION PROCESSES
STAINS
STRUCTURAL CHEMICAL ANALYSIS
VELOCITY
CONTROL EQUIPMENT
EQUIPMENT
FLUIDIC DEVICES
LUMINESCENCE
SIZE
550200* - Biochemistry
440300 - Miscellaneous Instruments- (-1989)