Effect of pregnancy or treatment with ethinylestradiol or phenobarbital on the glucuronidation of. beta. -estradiol at the C/sub 3/-OH vs C/sub 17/-OH in female rat liver microsomes
The glucuronidation of (/sup 3/H)-estradiol-17..beta.. (E/sub 2/) at the C/sub 3/ vs the C/sub 17/ hydroxyl groups was determined in female Sprague-Dawley rat liver microsomes. An HPLC method was developed to resolve the glucuronide conjugates which were then quantitated by liquid scintillation counting. The rates of formation of 17..beta..-estradiol 3-(..beta..-D-glucuronide) (E/sub 2/3G) and ..beta..-estradiol 17-(..beta..-D-glucuronide) (E/sub 2/17G) were 0.49 +- 0.03 and 0.40 +- 0.02 nmolminmg protein respectively. The apparent K/sub m/ and V/sub max/ of E/sub 2/ glucuronidation were determined in control, pregnant (day 19 of gestation), phenobarbital treated (PB; 80 mgkgday ip for 5 days) and ethinylestradiol treated (EE/sub 2/; 5 mgkgday ip for 5 days) female rats. Two methods were chosen for data analysis and the validity of these methods was compared. The least squares estimates of K/sub m/ and V/sub max/ values as well as the confidence contours of the joint sums of squares for the parameter spaces were calculated
- Research Organization:
- Kentucky Univ., Lexington (USA)
- OSTI ID:
- 5243896
- Resource Relation:
- Other Information: Thesis (Ph. D.)
- Country of Publication:
- United States
- Language:
- English
Similar Records
Characterization of multiple binding sites for /sup 3/H-Estradiol-17. beta. -(. beta. -D-glucuronide) in rat liver plasma membranes: evidence consonant with identification of an organic anion carrier
Direct radioimmunoassay of urinary estrogen and pregnanediol glucuronides during the menstrual cycle
Related Subjects
ESTRADIOL
BIOSYNTHESIS
GLUCURONIDE CONJUGATES
LEAST SQUARE FIT
LIQUID COLUMN CHROMATOGRAPHY
LIVER
MICROSOMES
PHENOBARBITAL
PREGNANCY
RATS
RESPONSE MODIFYING FACTORS
SCINTILLATION COUNTING
TRACER TECHNIQUES
TRITIUM COMPOUNDS
ANESTHETICS
ANIMALS
ANTICONVULSANTS
AZINES
BARBITURATES
BODY
CELL CONSTITUENTS
CENTRAL NERVOUS SYSTEM DEPRESSANTS
CHROMATOGRAPHY
COUNTING TECHNIQUES
DIGESTIVE SYSTEM
DRUGS
ESTRANES
ESTROGENS
GLANDS
HETEROCYCLIC COMPOUNDS
HORMONES
HYDROXY COMPOUNDS
HYPNOTICS AND SEDATIVES
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
MAMMALS
MAXIMUM-LIKELIHOOD FIT
METABOLITES
NUMERICAL SOLUTION
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC OXYGEN COMPOUNDS
ORGANOIDS
ORGANS
PYRIMIDINES
RODENTS
SEPARATION PROCESSES
STEROID HORMONES
STEROIDS
SYNTHESIS
VERTEBRATES
550201* - Biochemistry- Tracer Techniques