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Title: UV cross-linking of the Bacillus subtilis RNA polymerase to DNA in promoter and non-promoter complexes

Abstract

Complexes between Bacillus subtilus RNA polymerase and /sup 32/P-labeled DNA were irradiated with UV light and digested with nuclease; electrophoresis and autoradiography were used to identify the polymerase subunits cross-linked to DNA. These experiments showed: 1) that cross-linkage of promoter complexes yielded predominantly the beta and sigma subunits; 2) that beta, beta', and sigma were detected in non-promoter complexes; 3) that addition of the delta subunit or high concentrations of NaCl decreased cross-linkage of all subunits, especially the cross-linkage of the sigma subunit in non-promoter complexes and the binding of polymerase at DNA ends; 4) that different patterns of cross-linkage were obtained at 0 degrees C (conditions favoring the formation of closed complexes) and 37 degrees C (conditions favoring the formation of open complexes); and 5) predominantly beta and possibly alpha were cross-linked by irradiation of core-DNA complexes whereas similar experiments with core-delta complexed to DNA showed the efficient cross-linkage of beta' and beta.

Authors:
;
Publication Date:
Research Org.:
Univ. of Washington, Seattle
OSTI Identifier:
5243416
Resource Type:
Journal Article
Journal Name:
J. Biol. Chem.; (United States)
Additional Journal Information:
Journal Volume: 13
Country of Publication:
United States
Language:
English
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; DNA; GENETIC RADIATION EFFECTS; RNA POLYMERASES; CROSS-LINKING; AUTORADIOGRAPHY; BACILLUS SUBTILIS; BACTERIOPHAGES; CHEMICAL BONDS; ELECTROPHORESIS; LABELLED COMPOUNDS; PHOSPHORUS 32; PLASMIDS; SODIUM CHLORIDES; TRACER TECHNIQUES; ULTRAVIOLET RADIATION; ALKALI METAL COMPOUNDS; BACILLUS; BACTERIA; BETA DECAY RADIOISOTOPES; BETA-MINUS DECAY RADIOISOTOPES; BIOLOGICAL EFFECTS; BIOLOGICAL RADIATION EFFECTS; CELL CONSTITUENTS; CHEMICAL REACTIONS; CHLORIDES; CHLORINE COMPOUNDS; DAYS LIVING RADIOISOTOPES; ELECTROMAGNETIC RADIATION; ENZYMES; GENETIC EFFECTS; HALIDES; HALOGEN COMPOUNDS; ISOTOPE APPLICATIONS; ISOTOPES; LIGHT NUCLEI; MICROORGANISMS; NUCLEI; NUCLEIC ACIDS; NUCLEOTIDYLTRANSFERASES; ODD-ODD NUCLEI; ORGANIC COMPOUNDS; PARASITES; PHOSPHORUS ISOTOPES; PHOSPHORUS-GROUP TRANSFERASES; POLYMERASES; POLYMERIZATION; RADIATION EFFECTS; RADIATIONS; RADIOISOTOPES; SODIUM COMPOUNDS; TRANSFERASES; VIRUSES; 560112* - Radiation Effects on Biochemicals- In Microorganisms- (-1987)

Citation Formats

Hilton, M D, and Whiteley, H R. UV cross-linking of the Bacillus subtilis RNA polymerase to DNA in promoter and non-promoter complexes. United States: N. p., 1985. Web.
Hilton, M D, & Whiteley, H R. UV cross-linking of the Bacillus subtilis RNA polymerase to DNA in promoter and non-promoter complexes. United States.
Hilton, M D, and Whiteley, H R. 1985. "UV cross-linking of the Bacillus subtilis RNA polymerase to DNA in promoter and non-promoter complexes". United States.
@article{osti_5243416,
title = {UV cross-linking of the Bacillus subtilis RNA polymerase to DNA in promoter and non-promoter complexes},
author = {Hilton, M D and Whiteley, H R},
abstractNote = {Complexes between Bacillus subtilus RNA polymerase and /sup 32/P-labeled DNA were irradiated with UV light and digested with nuclease; electrophoresis and autoradiography were used to identify the polymerase subunits cross-linked to DNA. These experiments showed: 1) that cross-linkage of promoter complexes yielded predominantly the beta and sigma subunits; 2) that beta, beta', and sigma were detected in non-promoter complexes; 3) that addition of the delta subunit or high concentrations of NaCl decreased cross-linkage of all subunits, especially the cross-linkage of the sigma subunit in non-promoter complexes and the binding of polymerase at DNA ends; 4) that different patterns of cross-linkage were obtained at 0 degrees C (conditions favoring the formation of closed complexes) and 37 degrees C (conditions favoring the formation of open complexes); and 5) predominantly beta and possibly alpha were cross-linked by irradiation of core-DNA complexes whereas similar experiments with core-delta complexed to DNA showed the efficient cross-linkage of beta' and beta.},
doi = {},
url = {https://www.osti.gov/biblio/5243416}, journal = {J. Biol. Chem.; (United States)},
number = ,
volume = 13,
place = {United States},
year = {Fri Jul 05 00:00:00 EDT 1985},
month = {Fri Jul 05 00:00:00 EDT 1985}
}