Human cDNA clones for an. cap alpha. subunit of G/sub i/ signal-transduction protein

Journal Article · Sat Aug 01 00:00:00 EDT 1987 · Proc. Natl. Acad. Sci. U.S.A.; (United States)

DOI:https://doi.org/10.1073/pnas.84.15.5115· OSTI ID:5242991

Bray, P; Carter, A; Guo, V; Puckett, C; Kamholz, J; Spiegel, A; Nirenberg, M

Two cDNA clones were obtained from a lambdagt11 cDNA human brain library that correspond to ..cap alpha../sub i/ subunits of G signal-transduction proteins (where ..cap alpha../sub i/ subunits refer to the ..cap alpha.. subunits of G proteins that inhibit adenylate cyclase). The nucleotide sequence of human brain ..cap alpha../sub i/ is highly homologous to that of bovine brain ..cap alpha../sub i/ and the predicted amino acid sequences are identical. However, human and bovine brain ..cap alpha../sub i/ cDNAs differ significantly from ..cap alpha../sub i/ cDNAs from human monocytes, rat glioma, and mouse macrophages in amino acid (88% homology) and nucleotide (71-75% homology) sequences. In addition, the nucleotide sequences of the 3' untranslated regions of human and bovine brain ..cap alpha../sub i/ cDNAs differ markedly from the sequences of human monocyte, rat glioma, and mouse macrophage ..cap alpha../sub i/ cDNAs. These results suggest there are at least two classes of ..cap alpha../sub i/ mRNA

• Research Organization: National Institutes of Health, Bethesda, MD (USA)
• OSTI ID: 5242991
• Journal Information: Proc. Natl. Acad. Sci. U.S.A.; (United States), Vol. 84:15
• Country of Publication: United States
• Language: English

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59 BASIC BIOLOGICAL SCIENCES
MESSENGER-RNA
AUTORADIOGRAPHY
PROTEINS
AMINO ACID SEQUENCE
DNA-CLONING
BRAIN
CATTLE
CYCLASES
PHOSPHORUS 32
RECEPTORS
RECOMBINANT DNA
ANIMALS
BETA DECAY RADIOISOTOPES
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DOMESTIC ANIMALS
ENZYMES
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LIGHT NUCLEI
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MAMMALS
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MOLECULAR STRUCTURE
NERVOUS SYSTEM
NUCLEI
NUCLEIC ACIDS
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
ORGANS
PHOSPHORUS ISOTOPES
RADIOISOTOPES
RNA
RUMINANTS
VERTEBRATES
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