NF-kB activation by ultraviolet light not dependent on a nuclear signal

Journal Article · Fri Sep 10 00:00:00 EDT 1993 · Science (Washington, D.C.); (United States)

DOI:https://doi.org/10.1126/science.8367725· OSTI ID:5240414

Devary, Y; Rosette, C; DiDonato, J A; Karin, M ^[1]

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1. Univ. of California, San Diego, CA (United States)

Exposure of mammalian cells to radiation triggers the ultraviolet (UV) response, which includes activation of activator protein-1 (AP-1) and nuclear factor kappa B (NF-kB). This was postulated to occur by induction of a nuclear signaling cascade by damaged DNA. Recently, induction of AP-1 by UV was shown to be mediated by a pathway involving Src tyrosine kinases and the Ha-Ras small guanosine triphosphate-binding protein, proteins located at the plasma membrane. It is demonstrated here that the same pathway mediates induction of NF-kB by UV. Because inactive NF-kB is stored in the cytosol, analysis of its activation directly tests the involvement of a nuclear-initiated signaling cascade. Enucleated cells are fully responsive to UV both in NF-kB induction and in activation of another key signaling event. Therefore, the UV response does not require a signal generated in the nucleus and is likely to be initiated at or near the plasma membrane.

• DOE Contract Number: FG03-86ER60429
• OSTI ID: 5240414
• Journal Information: Science (Washington, D.C.); (United States), Vol. 261:5127; ISSN 0036-8075
• Country of Publication: United States
• Language: English

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