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Title: Interleukin-1 stimulates zinc uptake by human thymic epithelial cells

Abstract

Thymic epithelial cells (TEC) are known to secrete peptides which influence the differentiation and maturation of T-lymphocytes. These peptides include the thymic hormones thymulin, thymosin-{alpha}1, and thymopoietin. The biological activity of thymulin is dependent on the presence of zinc in an equimolar ratio. The authors have shown that both interleukin-1{alpha}(IL-1{alpha}) and interleukin-1{beta}(IL-1{beta}), which stimulate proliferation of TEC, stimulate the uptake of Zn-65 in-vitro independent of this proliferation. Mitomycin-C was used to inhibit the proliferation of TEC. Two other stimulators of proliferation of TEC, bovine pituitary extract (BPE) and epidermal growth factor (EGF), did not stimulate zinc uptake by the TEC independent of proliferation. They have also shown, utilizing in-situ hybridization, that IL-1 and zinc induce metallothionein(MT) mRNA expression in human thymic epithelial cells. The exact role of metallothionein is not clear, but it is thought to be involved in regulation of trace metal metabolism, especially in maintenance of zinc homeostasis. Their current hypothesis is that IL-1 stimulates uptake of zinc into the TEC, followed by its complexing with metallothionein. Zinc is then thought to be transferred from metallothionein to thymulin. Immunostaining, utilizing an antithymulin antibody and a fluoresceinated goat anti-rabbit second antibody, confirms the presence of thymulin in TEC andmore » its dependence on zinc. Upon stimulation, thymulin is then secreted. Known stimulants for thymulin include progesterone, dexamethasone, estradiol, testosterone, and prolactin. None of these secretagogues increase zinc uptake, suggesting the priming of the zinc-thymulin complex is unrelated to the regulation of its secretion.« less

Authors:
;  [1]
  1. Univ. of South Florida, Tampa (United States)
Publication Date:
OSTI Identifier:
5238894
Report Number(s):
CONF-9104107-
Journal ID: ISSN 0892-6638; CODEN: FAJOE
Resource Type:
Conference
Journal Name:
FASEB Journal (Federation of American Societies for Experimental Biology); (United States)
Additional Journal Information:
Journal Volume: 5:5; Conference: 75. annual meeting of the Federation of American Societies for Experimental Biology (FASEB), Atlanta, GA (United States), 21-25 Apr 1991; Journal ID: ISSN 0892-6638
Country of Publication:
United States
Language:
English
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; LYMPHOKINES; BIOCHEMICAL REACTION KINETICS; THYMUS CELLS; CELL PROLIFERATION; ZINC; MEMBRANE TRANSPORT; ANTIBODIES; EPITHELIUM; GROWTH FACTORS; HYBRIDIZATION; INHIBITION; MAN; MESSENGER-RNA; METABOLISM; METALLOTHIONEIN; MITOMYCIN; TRACER TECHNIQUES; ZINC 65; ANIMAL CELLS; ANIMAL TISSUES; ANIMALS; ANTI-INFECTIVE AGENTS; ANTIBIOTICS; ANTIMITOTIC DRUGS; ANTINEOPLASTIC DRUGS; BETA DECAY RADIOISOTOPES; BETA-PLUS DECAY RADIOISOTOPES; BODY; DAYS LIVING RADIOISOTOPES; DRUGS; ELECTRON CAPTURE RADIOISOTOPES; ELEMENTS; EVEN-ODD NUCLEI; INTERMEDIATE MASS NUCLEI; ISOTOPE APPLICATIONS; ISOTOPES; KINETICS; MAMMALS; METALLOPROTEINS; METALS; MITOGENS; NUCLEI; NUCLEIC ACIDS; ORGANIC COMPOUNDS; PRIMATES; PROTEINS; RADIOISOTOPES; REACTION KINETICS; RNA; SOMATIC CELLS; TISSUES; VERTEBRATES; ZINC ISOTOPES; 560300* - Chemicals Metabolism & Toxicology

Citation Formats

Coto, J A, and Hadden, J W. Interleukin-1 stimulates zinc uptake by human thymic epithelial cells. United States: N. p., 1991. Web.
Coto, J A, & Hadden, J W. Interleukin-1 stimulates zinc uptake by human thymic epithelial cells. United States.
Coto, J A, and Hadden, J W. 1991. "Interleukin-1 stimulates zinc uptake by human thymic epithelial cells". United States.
@article{osti_5238894,
title = {Interleukin-1 stimulates zinc uptake by human thymic epithelial cells},
author = {Coto, J A and Hadden, J W},
abstractNote = {Thymic epithelial cells (TEC) are known to secrete peptides which influence the differentiation and maturation of T-lymphocytes. These peptides include the thymic hormones thymulin, thymosin-{alpha}1, and thymopoietin. The biological activity of thymulin is dependent on the presence of zinc in an equimolar ratio. The authors have shown that both interleukin-1{alpha}(IL-1{alpha}) and interleukin-1{beta}(IL-1{beta}), which stimulate proliferation of TEC, stimulate the uptake of Zn-65 in-vitro independent of this proliferation. Mitomycin-C was used to inhibit the proliferation of TEC. Two other stimulators of proliferation of TEC, bovine pituitary extract (BPE) and epidermal growth factor (EGF), did not stimulate zinc uptake by the TEC independent of proliferation. They have also shown, utilizing in-situ hybridization, that IL-1 and zinc induce metallothionein(MT) mRNA expression in human thymic epithelial cells. The exact role of metallothionein is not clear, but it is thought to be involved in regulation of trace metal metabolism, especially in maintenance of zinc homeostasis. Their current hypothesis is that IL-1 stimulates uptake of zinc into the TEC, followed by its complexing with metallothionein. Zinc is then thought to be transferred from metallothionein to thymulin. Immunostaining, utilizing an antithymulin antibody and a fluoresceinated goat anti-rabbit second antibody, confirms the presence of thymulin in TEC and its dependence on zinc. Upon stimulation, thymulin is then secreted. Known stimulants for thymulin include progesterone, dexamethasone, estradiol, testosterone, and prolactin. None of these secretagogues increase zinc uptake, suggesting the priming of the zinc-thymulin complex is unrelated to the regulation of its secretion.},
doi = {},
url = {https://www.osti.gov/biblio/5238894}, journal = {FASEB Journal (Federation of American Societies for Experimental Biology); (United States)},
issn = {0892-6638},
number = ,
volume = 5:5,
place = {United States},
year = {Fri Mar 15 00:00:00 EST 1991},
month = {Fri Mar 15 00:00:00 EST 1991}
}

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