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Title: Laser microbeam irradiation and renucleation of mouse eggs. Final progress report, July 1, 1979-December 31, 1983

Abstract

We have combined laser microbeam irradiation of mouse egg nuclei with fusion to donor cell nuclei in order to develop a new procedure for transferring nuclei into mammalian eggs. We have been using virus-treated cells injected into the perivitelline for fusion with egg cells. Binucleate cells inside the zona pellucida were often produced indicating nuclear transfer between cells had occurred. To prevent the formation of such abortive polyploidy, host nuclei were inactivated with a laser microbeam. The subsequent cleavage of the microirradiated eggs has been studied.

Authors:
Publication Date:
Research Org.:
California Univ., San Francisco (USA). Dept. of Anatomy
OSTI Identifier:
5233793
Report Number(s):
DOE/EV/70289-T2
ON: DE84006666
DOE Contract Number:  
AT03-76EV70289
Resource Type:
Technical Report
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; CELL NUCLEI; BIOLOGICAL RADIATION EFFECTS; TRANSPLANTS; OOCYTES; LASERS; MICE; MICROSCOPY; ONTOGENESIS; ANIMALS; BIOLOGICAL EFFECTS; CELL CONSTITUENTS; GERM CELLS; MAMMALS; RADIATION EFFECTS; RODENTS; VERTEBRATES; 550800* - Morphology

Citation Formats

Lin, T P. Laser microbeam irradiation and renucleation of mouse eggs. Final progress report, July 1, 1979-December 31, 1983. United States: N. p., 1983. Web.
Lin, T P. Laser microbeam irradiation and renucleation of mouse eggs. Final progress report, July 1, 1979-December 31, 1983. United States.
Lin, T P. 1983. "Laser microbeam irradiation and renucleation of mouse eggs. Final progress report, July 1, 1979-December 31, 1983". United States.
@article{osti_5233793,
title = {Laser microbeam irradiation and renucleation of mouse eggs. Final progress report, July 1, 1979-December 31, 1983},
author = {Lin, T P},
abstractNote = {We have combined laser microbeam irradiation of mouse egg nuclei with fusion to donor cell nuclei in order to develop a new procedure for transferring nuclei into mammalian eggs. We have been using virus-treated cells injected into the perivitelline for fusion with egg cells. Binucleate cells inside the zona pellucida were often produced indicating nuclear transfer between cells had occurred. To prevent the formation of such abortive polyploidy, host nuclei were inactivated with a laser microbeam. The subsequent cleavage of the microirradiated eggs has been studied.},
doi = {},
url = {https://www.osti.gov/biblio/5233793}, journal = {},
number = ,
volume = ,
place = {United States},
year = {Sat Jan 01 00:00:00 EST 1983},
month = {Sat Jan 01 00:00:00 EST 1983}
}

Technical Report:
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