Altered catecholamine receptor affinity in rabbit aortic intimal hyperplasia

Journal Article · Thu Aug 01 00:00:00 EDT 1991 · Journal of Surgical Research; (United States)

DOI:https://doi.org/10.1016/0022-4804(91)90086-2· OSTI ID:5227804

O'Malley, M K; Cotecchia, S; Hagen, P O ^[1]

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1. Duke University Medical Center, Durham, NC (USA)

Intimal thickening is a universal response to endothelial denudation and is also thought to be a precursor of atherosclerosis. The authors have demonstrated selective supersensitivity in arterial intimal hyperplasia to norepinephrine and they now report a possible mechanism for this. Binding studies in rabbit aorta with the selective alpha 1-adrenergic radioligand 125I-HEAT demonstrated that there was no change in receptor density (20 {plus minus} 4 fmole/10(6) cells) in intact vascular smooth muscle cells at either 5 or 14 days after denudation. However, competition studies showed a 2.6-fold increase in alpha 1-adrenergic receptor affinity for norepinephrine in intimal hyperplastic tissue (P less than 0.05). This increased affinity for norepinephrine was associated with a greater increase in 32P-labeled phosphatidylinositol (148% intimal thickening versus 76% control) and phosphatidic acid (151% intimal thickening versus 56% control) following norepinephrine stimulation of free floating rings of intimal hyperplastic aorta. These data suggest that the catecholamine supersensitivity in rabbit aortic intimal hyperplasia is receptor mediated and may be linked to the phosphatidylinositol cycle.

• OSTI ID: 5227804
• Journal Information: Journal of Surgical Research; (United States), Vol. 51:2; ISSN 0022-4804
• Country of Publication: United States
• Language: English

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59 BASIC BIOLOGICAL SCIENCES
CATECHOLAMINES
RECEPTORS
VASCULAR DISEASES
PATHOGENESIS
AFFINITY
AORTA
BIOCHEMICAL REACTION KINETICS
IODINE 125
LIGANDS
MUSCLES
NORADRENALINE
PHOSPHOLIPIDS
PHOSPHORUS 32
RABBITS
TRACER TECHNIQUES
ADRENAL HORMONES
AMINES
ANIMALS
AROMATICS
ARTERIES
AUTONOMIC NERVOUS SYSTEM AGENTS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BLOOD VESSELS
BODY
CARDIOTONICS
CARDIOVASCULAR AGENTS
CARDIOVASCULAR SYSTEM
DAYS LIVING RADIOISOTOPES
DISEASES
DRUGS
ELECTRON CAPTURE RADIOISOTOPES
ESTERS
HORMONES
HYDROXY COMPOUNDS
INTERMEDIATE MASS NUCLEI
INTERNAL CONVERSION RADIOISOTOPES
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
LIGHT NUCLEI
LIPIDS
MAMMALS
MEMBRANE PROTEINS
NEUROREGULATORS
NUCLEI
ODD-EVEN NUCLEI
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
ORGANS
PHENOLS
PHOSPHORUS ISOTOPES
POLYPHENOLS
PROTEINS
RADIOISOTOPES
REACTION KINETICS
SYMPATHOMIMETICS
VERTEBRATES
550901* - Pathology- Tracer Techniques