Characterization of a putative acetylcholine receptor in chick ciliary ganglion neurons
Monoclonal antibodies to the main immunogenic region on the alpha subunit of acetylcholine receptors in muscle and electric organ recognize membrane components in chick brain and ciliary ganglia that are candidates for the neuronal receptor. The component in chick brain has been purified by immunoaffinity chromatography. It specifically binds nicotine but not alpha-bungarotoxin, and can be affinity labeled with (/sup 3/H)bromoacetylcholine. The cross-reacting component in ciliary ganglion neurons is concentrated in synaptic membrane, and can be modulated by exposure of the cells to cholinergic ligands in culture. The cross-reacting component in ciliary ganglion neurons is an integral membrane component that binds concanavalin A, and it is distinct from the alpha-bungarotoxin binding component. The acetylcholine receptor function in these neurons can be locked by affinity alkylation with bromoacetylcholine, indicating similarity in this respect to receptors from muscle and electric organ. Antisera raised against the partially purified component from chick brain also block receptor function on ciliary ganglion neurons. The subcellular distribution of the ganglion component in culture is assessed, and it is shown that approximately 2/3 of the cross-reacting components are intracellular; the majority of these seem not to be destined for insertion into the plasma membrane.
- Research Organization:
- California Univ., San Diego (USA)
- OSTI ID:
- 5219891
- Resource Relation:
- Other Information: Thesis (Ph. D.)
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
ACETYLCHOLINE
BIOCHEMICAL REACTION KINETICS
RADIORECEPTOR ASSAY
RECEPTORS
NERVE CELLS
ALKYLATION
BRAIN
CELL MEMBRANES
CHICKENS
CHROMATOGRAPHY
CONCANAVALIN
EXPERIMENTAL DATA
GANGLIONS
IMMUNE SERUMS
LIGANDS
MONOCLONAL ANTIBODIES
NICOTINE
ORGANIC BROMINE COMPOUNDS
PURIFICATION
TRACER TECHNIQUES
TRITIUM COMPOUNDS
AGGLUTININS
ALKALOIDS
AMINES
AMMONIUM COMPOUNDS
ANIMAL CELLS
ANIMALS
ANTIBODIES
AUTONOMIC NERVOUS SYSTEM AGENTS
AZINES
AZOLES
BIRDS
BODY
CELL CONSTITUENTS
CENTRAL NERVOUS SYSTEM
CHEMICAL REACTIONS
DATA
DRUGS
ESTERS
FOWL
HEMAGGLUTININS
HETEROCYCLIC COMPOUNDS
INFORMATION
ISOTOPE APPLICATIONS
KINETICS
LABELLED COMPOUNDS
LECTINS
MEMBRANE PROTEINS
MEMBRANES
NERVOUS SYSTEM
NEUROREGULATORS
NUMERICAL DATA
ORGANIC COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
PARASYMPATHOLYTICS
PARASYMPATHOMIMETICS
PROTEINS
PYRIDINES
PYRROLES
PYRROLIDINES
QUATERNARY COMPOUNDS
REACTION KINETICS
SEPARATION PROCESSES
SOMATIC CELLS
VERTEBRATES
550201* - Biochemistry- Tracer Techniques