Biological activities of indoleacetylamino acids and their use as auxins in tissue culture
The auxin activities of a number of indoleacetylamino acid conjugates have been determined in three test systems: growth of tomato hypocotyl explants (Lycopersicon esculentum Mill. cv. Marglobe); growth of tobacco callus cultures (Nicotiana tabacum L. cv. Wisconsin 38); and ethylene production from pea stems (Pisum sativum L. cv. Alaska). The activities of the conjugates differ greatly depending on the amino acid moiety. Indoleacetyl-L-alanine supports rapid callus growth from the tomato hypocotyls while inhibiting growth of shoots and roots. Indoleacetlyglycine behaves in a similar manner but is somewhat less effective in supporting callus growth and in inhibiting growth of shoots and roots. Indoleacetylglycine behaves in a similar manner but is somewhat less effective in supporting callus growth and in inhibiting shoot formation. The other amino acid conjugates tested (valine, leucine, aspartic acid, threonine, methionine, phenylalanine, and proline) support shoot formation without supporting root formation or much callus growth. The tobacco callus system, which forms abundant shoots in the presence or absence of free indoleacetic acid, produces only rapid undifferentiated growth in the presence of indoleacetyl-L-alanine and indoleacetylglycine. The other conjugates inhibit shoot formatin weakly if at all. Most of the conjugates induce sustained ethylene production from the pea stems but at rates well below the initial rates observed with free indoleacetic acid. Many, but not all of the effects of conjugates such as indoleacetyl-L-alanine can be mimicked by frequent renewals of the supply of free indoleacetic acid.
- Research Organization:
- Michigan State Univ., East Lansing, MI (United States)
- DOE Contract Number:
- EY-76-S-02-2528M005
- OSTI ID:
- 5213090
- Journal Information:
- Plant Physiol.; (United States), Vol. 65:5
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
AMINO ACIDS
BIOLOGICAL EFFECTS
AUXINS
INDOLES
NICOTIANA
PLANT GROWTH
SENSITIVITY
PISUM
TOMATOES
BIOCHEMISTRY
INHIBITION
PLANT STEMS
ROOTS
TISSUE CULTURES
AZOLES
CARBOXYLIC ACIDS
CHEMISTRY
FOOD
GROWTH
HETEROCYCLIC COMPOUNDS
LEGUMINOSAE
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PLANT GROWTH REGULATORS
PLANTS
PYRROLES
VEGETABLES
550200* - Biochemistry
550300 - Cytology