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Title: Direct radioimmunoassay of urinary estrogen and pregnanediol glucuronides during the menstrual cycle

Abstract

Assays measuring immunoreactive estrone glucuronide (E/sub 1/G), estradiol-3-glucuronide (E/sub 2/-3G), estradiol-17..beta..-glucuronide (E/sub 2/-17G), estriol-3-glucuronide (E/sub 3/-3G), estriol-16..cap alpha..-glucuronide (E/sub 3/-16G), and pregnanediol-3..cap alpha..-glucuronide (Pd-3G) directly in diluted urine were developed and validated. These estrogen and pregnanediol glucuronide fractions were measured in aliquots of 24-hour and overnight samples of urine collected daily from seven women for one menstrual cycle. Urinary hormone excretion was correlated with daily serum estradiol (E/sub 2/), progesterone (P), and lutenizing hormonee (LH) levels. A sharp midcycle LH peak preceded by a preovulatory rise in serum E/sub 2/ and followed by luteal phase serum P levels were noted in each of the seven apparently ovulatory cycles. Twenty-four-hour and overnight urinary excretion patterns of estrogen glucuronides were similar to those of serum E/sub 2/. Of the five estrogen glucuronide fractions tested, excretion of E/sub 2/-17G exhibited the earliest and steepest ascending slope of the preovulatory estrogen surge and correlated best with serum E/sub 2/ levels. Urinary excretion of E/sub 1/-G, E/sub 2/-3G, and E/sub 3/-16G also showed an early and steep preovulatory rise and preceded that of E/sub 3/-3G, whereas urinary excretion of E/sub 3/-3G exhibited the poorest correlation with serum E/sub 2/ concentrations. The urinary excretion of Pd-3Gmore » rose parallel to serum P levels and was markedly elevated 2 to 3 days after the midcycle LH peak in both 24-hour and overnight collections of urine. These results indicate that among the urinary estrogen conjugate fractions tested, E/sub 2/-17G is the one that most suitably predicts ovulation.« less

Authors:
; ;
Publication Date:
OSTI Identifier:
5212489
Resource Type:
Journal Article
Journal Name:
Am. J. Obstet. Gynecol.; (United States)
Additional Journal Information:
Journal Volume: 137:4
Country of Publication:
United States
Language:
English
Subject:
62 RADIOLOGY AND NUCLEAR MEDICINE; 59 BASIC BIOLOGICAL SCIENCES; ESTROGENS; RADIOIMMUNOASSAY; COMPARATIVE EVALUATIONS; ESTROUS CYCLE; LABELLED COMPOUNDS; LH; OVULATION; TRITIUM; URINE; WOMEN; ANIMALS; BETA DECAY RADIOISOTOPES; BETA-MINUS DECAY RADIOISOTOPES; BIOLOGICAL MATERIALS; BIOLOGICAL WASTES; BODY FLUIDS; FEMALES; GONADOTROPINS; HORMONES; HYDROGEN ISOTOPES; ISOTOPE APPLICATIONS; ISOTOPES; LIGHT NUCLEI; MAMMALS; MAN; MATERIALS; NUCLEI; ODD-EVEN NUCLEI; PEPTIDE HORMONES; PITUITARY HORMONES; PRIMATES; RADIOASSAY; RADIOISOTOPES; STEROID HORMONES; TRACER TECHNIQUES; VERTEBRATES; WASTES; YEARS LIVING RADIOISOTOPES; 550601* - Medicine- Unsealed Radionuclides in Diagnostics; 551001 - Physiological Systems- Tracer Techniques

Citation Formats

Stanczyk, F.Z., Miyakawa, I., and Goebelsmann, U. Direct radioimmunoassay of urinary estrogen and pregnanediol glucuronides during the menstrual cycle. United States: N. p., 1980. Web.
Stanczyk, F.Z., Miyakawa, I., & Goebelsmann, U. Direct radioimmunoassay of urinary estrogen and pregnanediol glucuronides during the menstrual cycle. United States.
Stanczyk, F.Z., Miyakawa, I., and Goebelsmann, U. Sun . "Direct radioimmunoassay of urinary estrogen and pregnanediol glucuronides during the menstrual cycle". United States.
@article{osti_5212489,
title = {Direct radioimmunoassay of urinary estrogen and pregnanediol glucuronides during the menstrual cycle},
author = {Stanczyk, F.Z. and Miyakawa, I. and Goebelsmann, U.},
abstractNote = {Assays measuring immunoreactive estrone glucuronide (E/sub 1/G), estradiol-3-glucuronide (E/sub 2/-3G), estradiol-17..beta..-glucuronide (E/sub 2/-17G), estriol-3-glucuronide (E/sub 3/-3G), estriol-16..cap alpha..-glucuronide (E/sub 3/-16G), and pregnanediol-3..cap alpha..-glucuronide (Pd-3G) directly in diluted urine were developed and validated. These estrogen and pregnanediol glucuronide fractions were measured in aliquots of 24-hour and overnight samples of urine collected daily from seven women for one menstrual cycle. Urinary hormone excretion was correlated with daily serum estradiol (E/sub 2/), progesterone (P), and lutenizing hormonee (LH) levels. A sharp midcycle LH peak preceded by a preovulatory rise in serum E/sub 2/ and followed by luteal phase serum P levels were noted in each of the seven apparently ovulatory cycles. Twenty-four-hour and overnight urinary excretion patterns of estrogen glucuronides were similar to those of serum E/sub 2/. Of the five estrogen glucuronide fractions tested, excretion of E/sub 2/-17G exhibited the earliest and steepest ascending slope of the preovulatory estrogen surge and correlated best with serum E/sub 2/ levels. Urinary excretion of E/sub 1/-G, E/sub 2/-3G, and E/sub 3/-16G also showed an early and steep preovulatory rise and preceded that of E/sub 3/-3G, whereas urinary excretion of E/sub 3/-3G exhibited the poorest correlation with serum E/sub 2/ concentrations. The urinary excretion of Pd-3G rose parallel to serum P levels and was markedly elevated 2 to 3 days after the midcycle LH peak in both 24-hour and overnight collections of urine. These results indicate that among the urinary estrogen conjugate fractions tested, E/sub 2/-17G is the one that most suitably predicts ovulation.},
doi = {},
journal = {Am. J. Obstet. Gynecol.; (United States)},
number = ,
volume = 137:4,
place = {United States},
year = {1980},
month = {6}
}