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Title: Glucose metabolite patterns as markers of functional differentiation in freshly isolated and cultured mouse mammary epithelial cells

Abstract

In the mammary gland of non-ruminant animals, glucose is utilized in a characteristic and unique way during lacation. By measuring the incorporation of glucose carbon from (U-/sup 14/C)glucose into intermediary metabolitees and metabolic products in mammary epithelia cells from virgin, pregnant, and lacating mice, we domonstrate that glucose metabolite patterns can be used to recognize stages of differentiated function. For these cells, the rates of synthesis of glycogen and lactose, the ratio of lactate to alanine, and the ratio of citrate to malate are important parameters in identifying the degree of expression of differentiation. We further show that these patterns can be used as markers to determine the differentiated state of cultured mammary epithelial cells. Cells maintained on plastic substrates lose their distinctive glucose metabolite patterns while those on floating collagen gels do not. Cells isolated from pregnant mice and cultured on collagen gels have a pattern similar to that of their freshly isolated counter-parts. When isolated from lacating mice, the metabolite patterns of cells cultured on collagen gels are different from that of the cells of origin, and resembles that of freshly isolated cells from pregnant mice. Our findings suggest that the floating collagen gels under the culture conditionsmore » used in these experiments provide an environment for the functional expression of the pregnant state, while additional factors are needed for the expression of the lactating state.« less

Authors:
; ;
Publication Date:
Research Org.:
Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
OSTI Identifier:
5211964
DOE Contract Number:  
W-7405-ENG-48
Resource Type:
Journal Article
Journal Name:
Exp. Cell Res.; (United States)
Additional Journal Information:
Journal Volume: 134
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; CELL DIFFERENTIATION; BIOLOGICAL INDICATORS; GLUCOSE; METABOLITES; GLYCOGEN; BIOSYNTHESIS; LACTOSE; ANIMAL CELLS; CARBON 14 COMPOUNDS; CELL CULTURES; CITRATES; COLLAGEN; EPITHELIUM; LACTATION; MAMMARY GLANDS; MICE; PREGNANCY; ALDEHYDES; ANIMAL TISSUES; ANIMALS; BODY; CARBOHYDRATES; CARBOXYLIC ACID SALTS; DISACCHARIDES; GLANDS; HEXOSES; LABELLED COMPOUNDS; MAMMALS; MONOSACCHARIDES; OLIGOSACCHARIDES; ORGANIC COMPOUNDS; ORGANS; POLYSACCHARIDES; PROTEINS; RODENTS; SACCHARIDES; SCLEROPROTEINS; SYNTHESIS; TISSUES; VERTEBRATES; 550201* - Biochemistry- Tracer Techniques; 550301 - Cytology- Tracer Techniques

Citation Formats

Emerman, J T, Bartley, J C, and Bissel, M J. Glucose metabolite patterns as markers of functional differentiation in freshly isolated and cultured mouse mammary epithelial cells. United States: N. p., 1981. Web. doi:10.1016/0014-4827(81)90481-X.
Emerman, J T, Bartley, J C, & Bissel, M J. Glucose metabolite patterns as markers of functional differentiation in freshly isolated and cultured mouse mammary epithelial cells. United States. https://doi.org/10.1016/0014-4827(81)90481-X
Emerman, J T, Bartley, J C, and Bissel, M J. 1981. "Glucose metabolite patterns as markers of functional differentiation in freshly isolated and cultured mouse mammary epithelial cells". United States. https://doi.org/10.1016/0014-4827(81)90481-X.
@article{osti_5211964,
title = {Glucose metabolite patterns as markers of functional differentiation in freshly isolated and cultured mouse mammary epithelial cells},
author = {Emerman, J T and Bartley, J C and Bissel, M J},
abstractNote = {In the mammary gland of non-ruminant animals, glucose is utilized in a characteristic and unique way during lacation. By measuring the incorporation of glucose carbon from (U-/sup 14/C)glucose into intermediary metabolitees and metabolic products in mammary epithelia cells from virgin, pregnant, and lacating mice, we domonstrate that glucose metabolite patterns can be used to recognize stages of differentiated function. For these cells, the rates of synthesis of glycogen and lactose, the ratio of lactate to alanine, and the ratio of citrate to malate are important parameters in identifying the degree of expression of differentiation. We further show that these patterns can be used as markers to determine the differentiated state of cultured mammary epithelial cells. Cells maintained on plastic substrates lose their distinctive glucose metabolite patterns while those on floating collagen gels do not. Cells isolated from pregnant mice and cultured on collagen gels have a pattern similar to that of their freshly isolated counter-parts. When isolated from lacating mice, the metabolite patterns of cells cultured on collagen gels are different from that of the cells of origin, and resembles that of freshly isolated cells from pregnant mice. Our findings suggest that the floating collagen gels under the culture conditions used in these experiments provide an environment for the functional expression of the pregnant state, while additional factors are needed for the expression of the lactating state.},
doi = {10.1016/0014-4827(81)90481-X},
url = {https://www.osti.gov/biblio/5211964}, journal = {Exp. Cell Res.; (United States)},
number = ,
volume = 134,
place = {United States},
year = {Thu Jan 01 00:00:00 EST 1981},
month = {Thu Jan 01 00:00:00 EST 1981}
}