Agents that elevate the concentration of cAMP in platelets inhibits the formation of a NaDodSO/sub 4/-resistant complex between thrombin and a 40-kDa protein

Journal Article · Sat Aug 01 00:00:00 EDT 1987 · Proc. Natl. Acad. Sci. U.S.A.; (United States)

DOI:https://doi.org/10.1073/pnas.84.16.5620· OSTI ID:5208984

Lerea, K M; Glomset, J A

The authors studied the influence of prostaglandin E/sub 1/ and theophylline on the ability of rabbit or human platelets to form NaDodSO/sub 4/-resistant complexes between /sup 125/I-labeled thrombin and a platelet protein of approx. =40 kDa. Control platelets formed two types of these complexes, one that sedimented with the platelets and one that was found in the suspension medium. There were 30-40 sedimentable complexes per platelet and about three times this number of soluble complexes. Pretreatment of rabbit or human platelets with prostaglandin E/sub 1/ and theophylline decreased the formation of both types of complex by as much as 60-80%. The pretreatment was particularly effective when low doses of thrombin were used. When added to control platelets, such doses of thrombin caused the formation of sedimentable complexes and the concomitant formation of inositol trisphosphate. However, when added to platelets that had been pretreated with prostaglandin E/sub 1/ and theophylline, low doses of thrombin had little or no effect on either reaction.

• Research Organization: Univ. of Washington, Seattle (USA)
• OSTI ID: 5208984
• Journal Information: Proc. Natl. Acad. Sci. U.S.A.; (United States), Vol. 84:16
• Country of Publication: United States
• Language: English

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59 BASIC BIOLOGICAL SCIENCES
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THEOPHYLLINE
THROMBIN
AMP
COMPLEXES
ELECTROPHORESIS
IODINE 125
MAN
RABBITS
ANIMALS
AROMATICS
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BIOLOGICAL MATERIALS
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BODY FLUIDS
CARDIOVASCULAR AGENTS
COAGULANTS
DAYS LIVING RADIOISOTOPES
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ELECTRON CAPTURE RADIOISOTOPES
ENZYMES
HEMATOLOGIC AGENTS
HEMOSTATICS
HETEROCYCLIC COMPOUNDS
HYDROLASES
INTERMEDIATE MASS NUCLEI
IODINE ISOTOPES
ISOTOPES
MAMMALS
MATERIALS
NUCLEI
NUCLEOTIDES
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ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
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PEPTIDE HYDROLASES
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SERINE PROTEINASES
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VERTEBRATES
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