Prostacyclin (PGI sub 2 ) synthesis in basal and thrombin challenged aortic rings is depressed in copper deficient and copper marginal rats

Conference · Fri Mar 15 00:00:00 EST 1991 · FASEB Journal (Federation of American Societies for Experimental Biology); (United States)

OSTI ID:5202574

Nelson, S K; Mathias, M M; Allen, K G.D. ^[1]

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1. Colorado State Univ., Ft. Collins (United States) Dept. of Agriculture, Washington, DC (United States)

Thirty male weanling rats were fed AIN based copper deficient (CuD) diet containing 0.1% of the Cu chelator N,N{prime}-bis(2-aminoethyl)-1,3-propanediamine.4HCl for 1 week. Animals were then assigned to three groups of 10 each and fed copper adequate (CuA), copper marginal (CuM) on CuD diets providing 7.5, 1.6 and 0.5 {mu}g Cu/g respectively for 26 days. Animals were pair meal fed to CuD for the last 16 days. Thoracic aorta were excised and 2 mm rings incubated in 2 ml Krebs Henseleit salts, 10mM HEPES pH 7.4, 37{degree}, 95.5 O{sub 2}/CO{sub 2}. PGI{sub 2} in incubation medium was determined by RIA as 6-keto PGF{sub 1{alpha}}. Cu status was assessed by liver Cu and aortic tissue superoxide dismutase (CuSOD). PGI{sub 2} production by 1 hour rested rings was linear from 0 to 10 minutes. Rested aortic rings produced < 5% PGI{sub 2} as in freshly excised rings. PGI{sub 2} synthesis in basal incubation, was significantly depressed 41% in CuM and 56% in CuD aortic rings. Challenge with 1OU/ml thrombin significantly increased PGI{sub 2} synthesis in CuA, CuM and CuD aortic rings by 32%-54%. CuM and CuD aortic ring PGI{sub 2} synthesis was significantly depressed by 32% and 48% respectively in thrombin challenged incubations. Both CuM and CuD decrease basal and thrombin challenged aortic ring PGI{sub 2} synthesis and this is associated with changes in aortic tissue CuSOD.

• OSTI ID: 5202574
• Report Number(s): CONF-9104107-; CODEN: FAJOE
• Journal Information: FASEB Journal (Federation of American Societies for Experimental Biology); (United States), Vol. 5:5; Conference: 75. annual meeting of the Federation of American Societies for Experimental Biology (FASEB), Atlanta, GA (United States), 21-25 Apr 1991; ISSN 0892-6638
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
COPPER
BIOLOGICAL EFFECTS
PROSTAGLANDINS
BIOSYNTHESIS
THROMBIN
BIOCHEMICAL REACTION KINETICS
AORTA
METABOLISM
NUTRITIONAL DEFICIENCY
RADIOIMMUNOASSAY
SUPEROXIDE DISMUTASE
ARTERIES
BIOASSAY
BLOOD COAGULATION FACTORS
BLOOD VESSELS
BODY
CARDIOVASCULAR SYSTEM
COAGULANTS
DIAGNOSTIC TECHNIQUES
DRUGS
ELEMENTS
ENZYMES
HEMATOLOGIC AGENTS
HEMOSTATICS
HYDROLASES
IMMUNOASSAY
IMMUNOLOGY
ISOTOPE APPLICATIONS
KINETICS
METALS
ORGANIC COMPOUNDS
ORGANS
OXIDOREDUCTASES
PEPTIDE HYDROLASES
PROTEINS
RADIOASSAY
RADIOIMMUNODETECTION
RADIOIMMUNOLOGY
REACTION KINETICS
SERINE PROTEINASES
SYNTHESIS
TRACER TECHNIQUES
TRANSITION ELEMENTS
560300* - Chemicals Metabolism &amp; Toxicology