Part I. Metabolites of Penicillium verrucosum var. cyclopium; Part II. Regioselective deuteriations of deoxyribose and incorporation into deoxynucleosides
Penicillium verrucosum var. cyclopium is the fungus responsible for several outbreaks of neurotoxicoses among cattle in Tennessee beginning in 1979. Verrucosidin isolated from samples of P. verrucosum var. cyclopium was later shown to be a powerful neurotoxin capable of paralyzing its victims and was also said to cause tremoring in some cases. Part I of this dissertation describes the re-investigation of metabolites of P. verrucosum var. cyclopium and tremorgenicity of verrucosidin. Verrucosidin has been shown in this study to be non-tremorgenic. This research also describes development of efficient synthetic methods for incorporation of deuterium into the deoxyribose moiety of deoxyribonucleosides. Deuteriated deoxynucleosides are presently being considered for synthesis of deuteriated sequences of DNA to be utilized in {sup 1}H NMR studies of solution conformation and dynamics, as well as interactions with proteins, drugs, metals and carcinogens. A route for synthesizing 2-deoxy-D-ribose from D-ribonic-acid-{gamma}-lactone incorporating deuterium at the C-1, C-2 or C-5 positions is presented.
- Research Organization:
- Vanderbilt Univ., Nashville, TN (USA)
- OSTI ID:
- 5186893
- Resource Relation:
- Other Information: Thesis (Ph. D.)
- Country of Publication:
- United States
- Language:
- English
Similar Records
ISOTOPIC TRACER STUDIES ON THE BIOSYNTHESIS OF DEOXYRIBOSE IN THE RAT
Selective reversible deuteriation of oligodeoxynucleotides: simplification of two-dimensional nuclear Overhauser effect NMR spectra assignment of a non-self-complementary dodecamer duplex
Related Subjects
DEOXYRIBOSE
METABOLISM
METABOLITES
TOXICITY
NUCLEOSIDES
BIOSYNTHESIS
PENICILLIUM
CATTLE
DEUTERIUM
FUNGAL DISEASES
TRACER TECHNIQUES
ALDEHYDES
ANIMALS
CARBOHYDRATES
DISEASES
DOMESTIC ANIMALS
EUMYCOTA
FUNGI
HYDROGEN ISOTOPES
INFECTIOUS DISEASES
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
MAMMALS
MONOSACCHARIDES
NUCLEI
NUCLEOTIDES
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
PENTOSES
PLANTS
RIBOSIDES
RUMINANTS
SACCHARIDES
STABLE ISOTOPES
SYNTHESIS
VERTEBRATES
550501* - Metabolism- Tracer Techniques