Differential DNA sequence deletions from chromosomes 3, 11, 13, and 17 in squamous-cell carcinoma, large-cell carcinoma, and adenocarcinoma of the human lung

Journal Article · Sat Jul 01 00:00:00 EDT 1989 · Proceedings of the National Academy of Sciences of the United States of America; (USA)

DOI:https://doi.org/10.1073/pnas.86.13.5099· OSTI ID:5185854

Weston, A; Willey, J C; Modali, R; Sugimura, H; McDowell, E M; Resau, J; Light, B; Haugen, A; Mann, D L; Trump, B F; Harris, C C ^[1]

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1. National Institutes of Health, Bethesda, MD (USA)

Activation of protooncogens and inactivation of putative tumor suppressor genes are genetic lesions considered to be important in lung carcinogenesis. Fifty-four cases of non-small-cell lung cancer (23 adenocarcinomas, 23 squamous-cell carcinomas, and 8 large-cell carcinomas) were examined for loss of DNA sequences at 13 polymorphic genetic loci. Loss of heterozygosity was seen more frequently in squamous-cell carcinoma than in adenocarcinoma. The loss of DNA sequences from the short arm of chromosome 17 (D17S1 locus) was detected in 8 of 9 heterozygous cases of squamous-cell carcinoma and in only 2 of 11 heterozygous cases of adenocarcinomas. Loss of DNA sequences from chromosome 3 was seen in 16 of 31 cases where the constitutive DNA was heterozygous-i.e., informative. Loss of heterozygosity at the chromosome 13q locus, D13S3, was seen in 9 of 21 informative cases, and in 2 cases, both adenocarcinomas, duplication of the intact DNA sequences suggested the possibility that mitotic recombination had occurred. Frequent DNA sequence deletions, including those from chromosome 17, in squamous-cell carcinomas may reflect the extensive mutagenic and clastogenic effects of tobacco smoke that may lead to inactivation of putative tumor-suppressor genes.

• OSTI ID: 5185854
• Journal Information: Proceedings of the National Academy of Sciences of the United States of America; (USA), Vol. 86:13; ISSN 0027-8424
• Country of Publication: United States
• Language: English

Related Subjects

62 RADIOLOGY AND NUCLEAR MEDICINE
CARCINOMAS
CHROMOSOMAL ABERRATIONS
DNA
AUTORADIOGRAPHY
RFLPS
DNA SEQUENCING
HETEROCHROMOSOMES
LUNGS
MAN
ONCOGENES
PATHOGENESIS
PHOSPHORUS 32
TOBACCO SMOKES
AEROSOLS
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BODY
CHROMOSOMES
COLLOIDS
DAYS LIVING RADIOISOTOPES
DISEASES
DISPERSIONS
GENES
ISOTOPES
LIGHT NUCLEI
MAMMALS
MUTATIONS
NEOPLASMS
NUCLEI
NUCLEIC ACIDS
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
ORGANS
PHOSPHORUS ISOTOPES
PRIMATES
RADIOISOTOPES
RESIDUES
RESPIRATORY SYSTEM
SMOKES
SOLS
STRUCTURAL CHEMICAL ANALYSIS
VERTEBRATES
550601* - Medicine- Unsealed Radionuclides in Diagnostics