Copper uptake and retention in liver parenchymal cells isolated from nutritionally copper-deficient rats

Journal Article · Thu Aug 01 00:00:00 EDT 1991 · Journal of Nutrition; (United States)

OSTI ID:5185729

Van den Berg, G J; de Goeij, J J; Bock, I; Gijbels, M J; Brouwer, A; Lei, K Y; Hendriks, H F ^[1]

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1. Interfaculty Reactor Institute, Delft University of Technology (Netherlands)

Copper uptake and retention were studied in primary cultures of liver parenchymal cells isolated from copper-deficient rats. Male Sprague-Dawley rats were fed a copper-deficient diet (less than 1 mg Cu/kg) for 10 wk. Copper-deficient rats were characterized by low copper concentrations in plasma and liver, anemia, low plasma ceruloplasmin oxidase activity and increased 64Cu whole-body retention. Freshly isolated liver parenchymal cells from copper-deficient rats showed a higher 64Cu influx, which was associated with a higher apparent Vmax of 45 {plus minus} 4 pmol Cu.mg protein-1.min-1 as compared with 30 {plus minus} 3 pmol Cu.mg protein-1.min-1 for cells isolated from copper-sufficient rats. No significant difference in the apparent Km (approximately 30 mumol/L) was observed. Relative 64Cu efflux from cells from copper-deficient rats was significantly smaller than the efflux from cells from copper-sufficient rats after prelabeling as determined by 2-h efflux experiments. Analysis of the medium after efflux from cells from copper-deficient rats showed elevated protein-associated 64Cu, suggesting a higher incorporation of radioactive copper during metalloprotein synthesis. Effects of copper deficiency persist in primary cultures of parenchymal cells derived from copper-deficient rats, and short-term cultures of these cells offer a prospect for the study of cell biological aspects of the metabolic adaptation of the liver to copper deficiency.

• OSTI ID: 5185729
• Journal Information: Journal of Nutrition; (United States), Vol. 121:8; ISSN 0022-3166
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
59 BASIC BIOLOGICAL SCIENCES
COPPER
MEMBRANE TRANSPORT
LIVER CELLS
BIOLOGICAL ADAPTATION
OXIDOREDUCTASES
ENZYME ACTIVITY
CERULOPLASMIN
COPPER 64
METABOLISM
NUTRITIONAL DEFICIENCY
RATS
RETENTION
TRACER TECHNIQUES
ANIMAL CELLS
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BETA-PLUS DECAY RADIOISOTOPES
COMPLEXES
COPPER COMPLEXES
COPPER ISOTOPES
ELECTRON CAPTURE RADIOISOTOPES
ELEMENTS
ENZYMES
GLOBULINS
GLOBULINS-ALPHA
HOURS LIVING RADIOISOTOPES
INTERMEDIATE MASS NUCLEI
ISOTOPE APPLICATIONS
ISOTOPES
MAMMALS
METALLOPROTEINS
METALS
NUCLEI
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
PROTEINS
RADIOISOTOPES
RODENTS
SOMATIC CELLS
TRANSITION ELEMENT COMPLEXES
TRANSITION ELEMENTS
VERTEBRATES
560300* - Chemicals Metabolism & Toxicology
550501 - Metabolism- Tracer Techniques