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Title: Assignment of the murine protein kinase gene DLK to chromosome 15 in the vicinity of the bt/Koa locus by genetic linkage analysis

Abstract

We have cloned protein kinase genes from murine primordial germ cell-derived EG cells by a PCR-based strategy using degenerate primers corresponding to the conserved sequences in the catalytic domain of protein kinases. One of these clones, designated Gek2 (germ cell kinase 2), was used as a probe for screening of a mouse brain cDNA library and obtained clones contained an entire coding sequence. Comparison of the sequence of Gek2 with those in databases revealed that it was identical to a previously reported protein kinase gene, DLK. 8 refs., 1 fig.

Authors:
; ;  [1]
  1. Tokyo Univ. (Japan); and others
Publication Date:
OSTI Identifier:
518490
Resource Type:
Journal Article
Journal Name:
Genomics
Additional Journal Information:
Journal Volume: 40; Journal Issue: 2; Other Information: PBD: 1 Mar 1997
Country of Publication:
United States
Language:
English
Subject:
55 BIOLOGY AND MEDICINE, BASIC STUDIES; PHOSPHOTRANSFERASES; GENETIC MAPPING; DNA-CLONING; DNA SEQUENCING; CHROMOSOMES; PROTEINS; MICE; POLYMERASE CHAIN REACTION; PROBES; RFLPS; DNA HYBRIDIZATION; BIOLOGICAL MARKERS; MUTANTS

Citation Formats

Watanabe, Toshio, Yanagisawa, Masahiro, and Matsubara, Nobumichi. Assignment of the murine protein kinase gene DLK to chromosome 15 in the vicinity of the bt/Koa locus by genetic linkage analysis. United States: N. p., 1997. Web. doi:10.1006/geno.1996.4565.
Watanabe, Toshio, Yanagisawa, Masahiro, & Matsubara, Nobumichi. Assignment of the murine protein kinase gene DLK to chromosome 15 in the vicinity of the bt/Koa locus by genetic linkage analysis. United States. https://doi.org/10.1006/geno.1996.4565
Watanabe, Toshio, Yanagisawa, Masahiro, and Matsubara, Nobumichi. 1997. "Assignment of the murine protein kinase gene DLK to chromosome 15 in the vicinity of the bt/Koa locus by genetic linkage analysis". United States. https://doi.org/10.1006/geno.1996.4565.
@article{osti_518490,
title = {Assignment of the murine protein kinase gene DLK to chromosome 15 in the vicinity of the bt/Koa locus by genetic linkage analysis},
author = {Watanabe, Toshio and Yanagisawa, Masahiro and Matsubara, Nobumichi},
abstractNote = {We have cloned protein kinase genes from murine primordial germ cell-derived EG cells by a PCR-based strategy using degenerate primers corresponding to the conserved sequences in the catalytic domain of protein kinases. One of these clones, designated Gek2 (germ cell kinase 2), was used as a probe for screening of a mouse brain cDNA library and obtained clones contained an entire coding sequence. Comparison of the sequence of Gek2 with those in databases revealed that it was identical to a previously reported protein kinase gene, DLK. 8 refs., 1 fig.},
doi = {10.1006/geno.1996.4565},
url = {https://www.osti.gov/biblio/518490}, journal = {Genomics},
number = 2,
volume = 40,
place = {United States},
year = {Sat Mar 01 00:00:00 EST 1997},
month = {Sat Mar 01 00:00:00 EST 1997}
}