SMT3A, a human homologue of the S. cerevisiae SMT3 gene, maps to chromosome 21qter and defines a novel gene family
- Catholic Univ., Rome (Italy)
- Osaka, Univ. (Japan); and others
cDNA selection was used to isolate coding sequences from cosmids mapping to the gene-rich telomeric region of human chromosome 21q. A novel cDNA, termed SMT3A, was isolated and mapped between the loci PFKL and D21S171, about 2.2 Mb proximal to the telomere. The predicted protein of 103 amino acids appears to be a homologue of the Saccharomyces cerevisiae SMT3 protein, whose gene was previously isolated as a suppressor of mutations in the MIF2 gene. The yeast MIF2 gene encodes an essential centromeric protein and shows homology to mammalian CENP-C, an integral component of active kinetochores. SMT3A was found to be highly homologous to two other recently isolated human genes, suggesting the presence of a new gene family. Homologous sequences were also found in protozoa, metazoa, and plants. Moreover, all predicted proteins show significant homology to ubiquitin. The proposed role of yeast SMT3 as centromeric protein and the strong evolutionary conservation of the SMT3A gene suggest an involvement of the encoded protein in the function and/or structure of the eukaryotic kinetochore. 30 refs., 5 figs.
- OSTI ID:
- 518487
- Journal Information:
- Genomics, Vol. 40, Issue 2; Other Information: PBD: 1 Mar 1997
- Country of Publication:
- United States
- Language:
- English
Similar Records
Localization of a human homolog of the mouse pericentrin gene (PCNT) to chromosome 21qter
A novel Creb family gene telomeric of HLA-DRA in the HLA complex
Related Subjects
BASIC STUDIES
GENES
GENETIC MAPPING
DNA SEQUENCING
GENE MUTATIONS
BIOLOGICAL EVOLUTION
DNA-CLONING
RESOLUTION
HUMAN CHROMOSOME 21
CHROMOSOMAL ABERRATIONS
DOWNS SYNDROME
ETIOLOGY
SACCHAROMYCES CEREVISIAE
BIOLOGICAL MARKERS
AMINO ACID SEQUENCE
HEREDITARY DISEASES
NUCLEOTIDES
DNA HYBRIDIZATION
MENTAL DISORDERS
EPILEPSY
CONTIGS