Variation in sister chromatid exchange frequencies between human and pig whole blood, plasma leukocyte, and mononuclear leukocyte cultures
Sister chromatid exchange (SCE) induction by ultraviolet (UV) light was studied in both human and pig whole blood cultures (WBC) and plasma leukocyte cultures (PLC). No variation in SCE frequency was observed between pig WBC and PLC in control as well as in treated cells. Conversely, SCE frequencies of human PLC were consistently higher than those of WBC in control and UV-exposed cells. Thus, red blood cells (RBCs) do not influence the sensitivity of lymphocytes to UV LIGHT exposure, and there must be some different culture condition(s) in the inducation of SCEs between human WBC and PLC but not in swine lymphocyte cultures. Since the BrdUrd/lymphocyte ratio of WBC was halved in PLC, the effect of BrdUrd concentration in inducing the SCE baseline frequency of PLC may be ruled out. Neither the cell separation technique nor polymorphonuclear leukocytes had a significant role in the elevated SCE frequency of human PLC or MLC. Experiments where human RBCs were titrated into human PLC showed that the induction of an elevated SCE frequency of PLC was suppressed in a dose-dependent manner by the presence of RBCs in the culture medium. Since the incorporation of pig or human RBCs into human PLC as well as into MLC reduced the SCE frequency to that of WBC, a common component and/or function existing in these cells is suggested. Analysis of different RBC components showed that RBCs, specifically RBC ghosts, release a diffusible but not dialyzable corrective factor into culture medium that is able to reduce the SCE frequencies of PLC.
- Research Organization:
- Instituto Multidisciplinario de Biologica Celular (IMBICE), La Plata, Argentina
- OSTI ID:
- 5177424
- Journal Information:
- Environ. Mutagen.; (United States), Vol. 8:4
- Country of Publication:
- United States
- Language:
- English
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LEUKOCYTES
BIOLOGICAL RADIATION EFFECTS
SISTER CHROMATID EXCHANGES
MUTATION FREQUENCY
RADIOINDUCTION
BLOOD COUNT
CELL CULTURES
ERYTHROCYTES
EXTREME ULTRAVIOLET RADIATION
MAN
SWINE
ANIMALS
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CHROMOSOMAL ABERRATIONS
DOMESTIC ANIMALS
ELECTROMAGNETIC RADIATION
MAMMALS
MATERIALS
MUTATIONS
PRIMATES
RADIATION EFFECTS
RADIATIONS
ULTRAVIOLET RADIATION
VERTEBRATES
560121* - Radiation Effects on Cells- External Source- (-1987)