Four RFLPs of the human insulin receptor gene: PstI, KpnI, RsaI (2 RFLPs)

Journal Article · Wed Jan 25 00:00:00 EST 1989 · Nucleic Acids Research; (UK)

DOI:https://doi.org/10.1093/nar/17.2.820· OSTI ID:5162614

Cox, N J; Spielman, R S; Taub, R ^[1]; Kahn, C R; Muller-Wieland, D; Kriauciunas, K M ^[2]

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1. Univ. of Pennsylvania School of Medicine, Philadelphia (USA)
2. Harvard Medical School, Boston, MA (USA)

Fragments were isolated from subclones containing the human insulin receptor cDNA. Probe 1 was a 677 bp XhoI/EcoRI fragment from the {alpha}-subunit region of the insulin receptor cDNA corresponding to nucleotides 334 to 1,011, the putative ligand binding domain. Probe 2 was a 1,599 bp PstI fragment from the {beta}-subunit region of the insulin receptor cDNA corresponding to nucleotides 2,746 to 4,345, encoding the tyrosine kinase domain.

• OSTI ID: 5162614
• Journal Information: Nucleic Acids Research; (UK), Vol. 17:2; ISSN 0305-1048
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
INSULIN
RECEPTORS
RFLPS
DNA SEQUENCING
GENES
LIGANDS
MAN
RECOMBINANT DNA
TYROSINASE
ANIMALS
DNA
ENZYMES
HORMONES
HYDROXYLASES
MAMMALS
MEMBRANE PROTEINS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
OXIDOREDUCTASES
PEPTIDE HORMONES
PRIMATES
PROTEINS
STRUCTURAL CHEMICAL ANALYSIS
VERTEBRATES
550200* - Biochemistry