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Title: Radiometric assay for determining the incorporation of L-canavanine or L-arginine into protein

Abstract

Procedures for a radiometric assay of L-(guanidinooxy-/sup 14/C)canavanine were developed which provide a convenient and accurate measure of the incorporation of (/sup 14/C)canavanine into de novo-synthesized proteins. These methods are also applicable to determining (/sup 14/C)arginine incorporation into protein. These procedures have been employed to study the synthesis of L-(guanidinooxy-/sup 14/C)canavanine- and L-(guanidino-/sup 14/C)arginine-containing proteins from the hemolymph of Manduca sexta and Heliothis virescens, two highly destructive insect pests.

Authors:
;
Publication Date:
Research Org.:
Univ. of Kentucky, Lexington
OSTI Identifier:
5151554
Resource Type:
Journal Article
Journal Name:
Anal. Biochem.; (United States)
Additional Journal Information:
Journal Volume: 2
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; PROTEINS; BIOSYNTHESIS; ARGININE; CARBON 14 COMPOUNDS; INSECTS; RADIOASSAY; TRACER TECHNIQUES; AMINO ACIDS; ANIMALS; ARTHROPODS; CARBOXYLIC ACIDS; INVERTEBRATES; ISOTOPE APPLICATIONS; LABELLED COMPOUNDS; ORGANIC ACIDS; ORGANIC COMPOUNDS; SYNTHESIS; 550201* - Biochemistry- Tracer Techniques

Citation Formats

Rosenthal, G A, and Thomas, D A. Radiometric assay for determining the incorporation of L-canavanine or L-arginine into protein. United States: N. p., 1985. Web. doi:10.1016/0003-2697(85)90292-1.
Rosenthal, G A, & Thomas, D A. Radiometric assay for determining the incorporation of L-canavanine or L-arginine into protein. United States. https://doi.org/10.1016/0003-2697(85)90292-1
Rosenthal, G A, and Thomas, D A. 1985. "Radiometric assay for determining the incorporation of L-canavanine or L-arginine into protein". United States. https://doi.org/10.1016/0003-2697(85)90292-1.
@article{osti_5151554,
title = {Radiometric assay for determining the incorporation of L-canavanine or L-arginine into protein},
author = {Rosenthal, G A and Thomas, D A},
abstractNote = {Procedures for a radiometric assay of L-(guanidinooxy-/sup 14/C)canavanine were developed which provide a convenient and accurate measure of the incorporation of (/sup 14/C)canavanine into de novo-synthesized proteins. These methods are also applicable to determining (/sup 14/C)arginine incorporation into protein. These procedures have been employed to study the synthesis of L-(guanidinooxy-/sup 14/C)canavanine- and L-(guanidino-/sup 14/C)arginine-containing proteins from the hemolymph of Manduca sexta and Heliothis virescens, two highly destructive insect pests.},
doi = {10.1016/0003-2697(85)90292-1},
url = {https://www.osti.gov/biblio/5151554}, journal = {Anal. Biochem.; (United States)},
number = ,
volume = 2,
place = {United States},
year = {Sat Jun 01 00:00:00 EDT 1985},
month = {Sat Jun 01 00:00:00 EDT 1985}
}