Biosynthesis of methylphosphomannosyl residues in the oligosaccharides of Dictyostelium discoideum glycoproteins: evidence that the methyl group is derived from methionine
The phosphorylated oligosaccharides of Dictyostelium discoideum contain methylphosphomannosyl residues which are stable to mild-acid and base hydrolysis. Evidence is presented that these methyl groups are derived from (methyl-/sup 3/H)methionine, in vivo and (methyl-/sup 3/H)S-adenosylmethionine in vitro. About 18% of the macromolecules secreted from vegetative cells labeled with (methyl-/sup 3/H)methionine are released by digestion with preparations of endoglycosidase/peptide N-glycosidase F. The majority of the released molecules are sulfated, anionic high mannose-type oligosaccharides. Strong acid hydrolysis of the (/sup 3/H)methyl-labeled molecules yields (/sup 3/H)methanol with kinetics of release similar to those found for the generation of Man-6-P from chemically synthesized methylphosphomannose methylglycoside. Treatment of the (/sup 3/H)methyl-labeled molecules with a phosphodiesterase from Aspergillus niger which is known to cleave this phosphodiester also releases (/sup 3/H)methanol from a portion of the oligosaccharides.
- Research Organization:
- Univ. of California, San Diego, Ja Jolla
- OSTI ID:
- 5150778
- Journal Information:
- J. Biol. Chem.; (United States), Vol. 261:1
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
FUNGI
GLUCOPROTEINS
OLIGOSACCHARIDES
BIOCHEMICAL REACTION KINETICS
BIOSYNTHESIS
ASPERGILLUS
EXPERIMENTAL DATA
GLYCOSYL HYDROLASES
HYDROLYSIS
IN VITRO
IN VIVO
MANNOSE
METHANOL
METHIONINE
PHOSPHODIESTERASES
TRACER TECHNIQUES
TRITIUM COMPOUNDS
ALCOHOLS
ALDEHYDES
AMINO ACIDS
CARBOHYDRATES
CARBOXYLIC ACIDS
CHEMICAL REACTIONS
DATA
DECOMPOSITION
DRUGS
ENZYMES
ESTERASES
HEXOSES
HYDROLASES
HYDROXY COMPOUNDS
INFORMATION
ISOTOPE APPLICATIONS
KINETICS
LABELLED COMPOUNDS
LIPOTROPIC FACTORS
LYSIS
MONOSACCHARIDES
NUMERICAL DATA
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
PLANTS
PROTEINS
REACTION KINETICS
SACCHARIDES
SOLVOLYSIS
SYNTHESIS
550201* - Biochemistry- Tracer Techniques
550701 - Microbiology- Tracer Techniques