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Title: Unknown sequence amplification: Application to in vitro genome walking in Chlamydia trachomatis L2

Abstract

A recently described technique, Chemical Genetics' unknown sequence amplification method, which requires only one specific oligonucleotide, has broadened the applicability of the polymerase chain reaction to DNA of unknown sequence. The authors have adapted this technique to the study of the genome of Chlamydia trachomatis, an obligate intracellular bacterium, and describe modifications that significantly improve the utility of this approach. These techniques allow for rapid genomic analysis entirely in vitro, using DNA of limited quantity of purity.

Authors:
; ; ;  [1]
  1. (ICI Diagnostics, Cheshire (United Kingdom))
Publication Date:
OSTI Identifier:
5148111
Resource Type:
Journal Article
Resource Relation:
Journal Name: Bio/Technology; (United States); Journal Volume: 9:1
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; DNA SEQUENCING; BACTERIA; DNA POLYMERASES; GENE AMPLIFICATION; OLIGONUCLEOTIDES; ENZYMES; MICROORGANISMS; NUCLEIC ACIDS; NUCLEOTIDYLTRANSFERASES; ORGANIC COMPOUNDS; PHOSPHORUS-GROUP TRANSFERASES; POLYMERASES; PROTEINS; STRUCTURAL CHEMICAL ANALYSIS; TRANSFERASES; 550400* - Genetics; 550700 - Microbiology

Citation Formats

Copley, C.G., Boot, C., Bundell, K., and McPheat, W.L. Unknown sequence amplification: Application to in vitro genome walking in Chlamydia trachomatis L2. United States: N. p., 1991. Web. doi:10.1038/nbt0191-74.
Copley, C.G., Boot, C., Bundell, K., & McPheat, W.L. Unknown sequence amplification: Application to in vitro genome walking in Chlamydia trachomatis L2. United States. doi:10.1038/nbt0191-74.
Copley, C.G., Boot, C., Bundell, K., and McPheat, W.L. Tue . "Unknown sequence amplification: Application to in vitro genome walking in Chlamydia trachomatis L2". United States. doi:10.1038/nbt0191-74.
@article{osti_5148111,
title = {Unknown sequence amplification: Application to in vitro genome walking in Chlamydia trachomatis L2},
author = {Copley, C.G. and Boot, C. and Bundell, K. and McPheat, W.L.},
abstractNote = {A recently described technique, Chemical Genetics' unknown sequence amplification method, which requires only one specific oligonucleotide, has broadened the applicability of the polymerase chain reaction to DNA of unknown sequence. The authors have adapted this technique to the study of the genome of Chlamydia trachomatis, an obligate intracellular bacterium, and describe modifications that significantly improve the utility of this approach. These techniques allow for rapid genomic analysis entirely in vitro, using DNA of limited quantity of purity.},
doi = {10.1038/nbt0191-74},
journal = {Bio/Technology; (United States)},
number = ,
volume = 9:1,
place = {United States},
year = {Tue Jan 01 00:00:00 EST 1991},
month = {Tue Jan 01 00:00:00 EST 1991}
}