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Title: Modulation of 2{prime}-5{prime} oligoadenylate synthetase by environmental stress in the marine sponge Geodia cydonium

Abstract

Recently the authors established the presence of relatively high amounts of 2{prime}-5{prime} oligoadenylates (2{prime}-5{prime} A) and 2{prime}-5{prime} oligoadenylate synthetase (2{prime}-5{prime} A synthetase) in the marine sponge Geodia cydonium. Here they determined by applying radioimmunoassay and high-performance liquid chromatographical methods that the concentration of 2{prime}-5{prime} A synthetase change following exposure of G. cydonium tissue to environmental stress. The 2{prime}-5{prime} A content and the activity of 2{prime}-5{prime} A synthetase, present in crude sponge extract, increase by up to three-fold after treating sponge cubes for 2 h with natural stressors including heat shock (26 C), cold shock (6 C), pH shock (pH 6), and hypertonic shock and subsequent incubation for 18 h under ambient conditions (16 C). No response was observed after exposure of sponges to an alkaline (pH 10) or hypotonic environment. Similar changes have been found for the expression of heat shock protein HSP70 in G. cydonium. These results show that 2{prime}-5{prime} A in sponges may be useful as a novel biomarker for environmental monitoring.

Authors:
; ;  [1]; ;  [2]
  1. Abteilung Angewandte Molekularbiologie, Mainz (Germany). Inst. fuer Physiologische Chemie
  2. Inst. of Chemical Physics and Biophysics, Tallinn (Estonia)
Publication Date:
Sponsoring Org.:
USDOE
OSTI Identifier:
514571
Resource Type:
Journal Article
Resource Relation:
Journal Name: Environmental Toxicology and Chemistry; Journal Volume: 16; Journal Issue: 7; Other Information: PBD: Jul 1997
Country of Publication:
United States
Language:
English
Subject:
54 ENVIRONMENTAL SCIENCES; 56 BIOLOGY AND MEDICINE, APPLIED STUDIES; BIOLOGICAL INDICATORS; WATER POLLUTION; BIOLOGICAL MARKERS; LIGASES; BIOLOGICAL STRESS; INVERTEBRATES; AQUATIC ORGANISMS; ENZYME ACTIVITY

Citation Formats

Schroeder, H.C., Wiens, M., Mueller, W.E.G., Kuusksalu, A., and Kelve, M. Modulation of 2{prime}-5{prime} oligoadenylate synthetase by environmental stress in the marine sponge Geodia cydonium. United States: N. p., 1997. Web. doi:10.1897/1551-5028(1997)016<1403:MOOSBE>2.3.CO;2.
Schroeder, H.C., Wiens, M., Mueller, W.E.G., Kuusksalu, A., & Kelve, M. Modulation of 2{prime}-5{prime} oligoadenylate synthetase by environmental stress in the marine sponge Geodia cydonium. United States. doi:10.1897/1551-5028(1997)016<1403:MOOSBE>2.3.CO;2.
Schroeder, H.C., Wiens, M., Mueller, W.E.G., Kuusksalu, A., and Kelve, M. 1997. "Modulation of 2{prime}-5{prime} oligoadenylate synthetase by environmental stress in the marine sponge Geodia cydonium". United States. doi:10.1897/1551-5028(1997)016<1403:MOOSBE>2.3.CO;2.
@article{osti_514571,
title = {Modulation of 2{prime}-5{prime} oligoadenylate synthetase by environmental stress in the marine sponge Geodia cydonium},
author = {Schroeder, H.C. and Wiens, M. and Mueller, W.E.G. and Kuusksalu, A. and Kelve, M.},
abstractNote = {Recently the authors established the presence of relatively high amounts of 2{prime}-5{prime} oligoadenylates (2{prime}-5{prime} A) and 2{prime}-5{prime} oligoadenylate synthetase (2{prime}-5{prime} A synthetase) in the marine sponge Geodia cydonium. Here they determined by applying radioimmunoassay and high-performance liquid chromatographical methods that the concentration of 2{prime}-5{prime} A synthetase change following exposure of G. cydonium tissue to environmental stress. The 2{prime}-5{prime} A content and the activity of 2{prime}-5{prime} A synthetase, present in crude sponge extract, increase by up to three-fold after treating sponge cubes for 2 h with natural stressors including heat shock (26 C), cold shock (6 C), pH shock (pH 6), and hypertonic shock and subsequent incubation for 18 h under ambient conditions (16 C). No response was observed after exposure of sponges to an alkaline (pH 10) or hypotonic environment. Similar changes have been found for the expression of heat shock protein HSP70 in G. cydonium. These results show that 2{prime}-5{prime} A in sponges may be useful as a novel biomarker for environmental monitoring.},
doi = {10.1897/1551-5028(1997)016<1403:MOOSBE>2.3.CO;2},
journal = {Environmental Toxicology and Chemistry},
number = 7,
volume = 16,
place = {United States},
year = 1997,
month = 7
}
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