Ferritin (FER), isoferritins and aluminum
FER from Alzheimer's brain contains more Al. One source of Al is beverages. Of the several common beverages analyzed, Pepsi, sold in bottles but not in cans, contained the highest conc. of Al (10..mu..M). Male albino rats were fed 10..mu..M Al in drinking water for one year. They were then sacrificed and their brain homogenates were analyzed for FER, Al and several enzymes. The results: compared to controls, the homogenates of the Al fed rats had 276.5% more Al bound to Fer (114.2 +/- 25.3 g atoms/mol) and 30% less hexokinase activity (150 units/mg protein). Acetyl choline esterase and alkaline ribonuclease levels remained unchanged. Isoelectrofocusing (pH 4-6.5) of human-brain FER yielded at least five bands. None corresponded with those from human liver FER or horse spleen FER. Horse spleen FER was applied to DEAE sephadex and eluted by NaCl-batchwise gradient. Five distinct fractions were obtained. The most acidic eluted last. It contained least Fe, tended to precipitate on standing and required less Al or Be to ppt. Thus, isoferritins may differ in their metal binding capacity and perhaps in their related physiological functions.
- Research Organization:
- Univ. of Tennessee, Knoxville
- OSTI ID:
- 5143503
- Report Number(s):
- CONF-8606151-
- Journal Information:
- Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Vol. 45:6; Conference: 76. annual meeting of the Federation of American Society for Experimental Biology, Washington, DC, USA, 8 Jun 1986
- Country of Publication:
- United States
- Language:
- English
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59 BASIC BIOLOGICAL SCIENCES
ALUMINIUM
BIOLOGICAL EFFECTS
TISSUE DISTRIBUTION
FERRITIN
BIOCHEMICAL REACTION KINETICS
MENTAL DISORDERS
PATHOGENESIS
ENZYME ACTIVITY
ESTERASES
HOMOGENATES
NUCLEASES
PHOSPHOTRANSFERASES
RATS
SPLEEN CELLS
ANIMAL CELLS
ANIMALS
COMPLEXES
DISTRIBUTION
ELEMENTS
ENZYMES
HYDROLASES
IRON COMPLEXES
KINETICS
MAMMALS
METALLOPROTEINS
METALS
ORGANIC COMPOUNDS
PHOSPHODIESTERASES
PHOSPHORUS-GROUP TRANSFERASES
PROTEINS
REACTION KINETICS
RODENTS
SOMATIC CELLS
TRANSFERASES
TRANSITION ELEMENT COMPLEXES
VERTEBRATES
560305* - Chemicals Metabolism & Toxicology- Vertebrates- (-1987)
550900 - Pathology