Induction of hepatic drug-metabolizing enzymes in rats treated with 1-nitropyrene

Journal Article · Mon Feb 01 00:00:00 EST 1988 · Environ. Res.; (United States)

DOI:https://doi.org/10.1016/S0013-9351(88)80011-2· OSTI ID:5142592

Belisario, M A; Borgia, R; Pecce, R; de Lorenzo, F

The inducing effects of 1-nitropyrene (1-NP) on the microsomal cytochrome P-450 system were studied in rats. Intraperitoneal administration of 1-NP led to increases in cytochrome P-450 content and aryl hydrocarbon hydroxylase, ethoxycoumarin, and ethoxyresorufin-O-deethylase activities. These increases were does dependent. Cytochrome b/sub 5/ content aminopyrine and p-nitroanisole demethylase activities were not affected by treatment of rats with 1-NP. Substrate specificity, sensitivity to mixed-function oxidase inhibitors, and electrophoretic pattern of 1-NP-induced cytochrome(s) P-450 were compared to the major forms of cytochrome P-450 induced by phenobarbital and methylcholanthrene. Furthermore microsomes from 1-NP-induced rats showed greater ability to metabolize the chemical as compared with those from control animals; this result indicates that 1-NP induces a form(s) of cytochrome P-450 especially effective in the metabolism of the substance itself.

• Research Organization: Istituto di Biochimica Cellulare e Molecolare, Napoli (Italy)
• OSTI ID: 5142592
• Journal Information: Environ. Res.; (United States), Vol. 45:1
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
CYTOCHROME OXIDASE
ENZYME ACTIVITY
HYDROXYLASES
POLYCYCLIC AROMATIC HYDROCARBONS
METABOLISM
BIOLOGICAL ADAPTATION
DOSE-RESPONSE RELATIONSHIPS
ENZYME INHIBITORS
METABOLIC ACTIVATION
METABOLITES
MICROSOMES
NITRO COMPOUNDS
RATS
ANIMALS
AROMATICS
CELL CONSTITUENTS
ENZYMES
HAEM DEHYDROGENASES
HYDROCARBONS
MAMMALS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANOIDS
OXIDOREDUCTASES
RODENTS
VERTEBRATES
550200* - Biochemistry