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Title: Activating mutations for transformation by p53 produce a gene product that forms an hsc70-p53 complex with an altered half-life

Abstract

The 11-4 p53 cDNA clone failed to transform primary rat fibroblasts when cotransfected with the ras oncogene. Two linker insertion mutations at amino acid 158 or 215 (of 390 amino acids) activated this p53 cDNA for transformation with ras. These mutant cDNAs produced a p53 protein that lacked an epitope, recognized by monoclonal antibody PAb246 (localized at amino acids 88 to 110 in the protein) and preferentially bound to a heat shock protein, hsc70. In rat cells transformed by a genomic p53 clone plus ras, two populations of p53 proteins were detected, PAb246/sup +/ and PAb246/sup -/, which did or did not bind to this monoclonal antibody, respectively. The PAb246/sup -/ p53 preferentially associated with hsc70, and this protein has a half-life 4- to 20-fold longer than free p53 (PAb246/sup +/). These data suggest a possible functional role for hsc70 in the transformation process. cDNAs for p53 derived from methylcholanthrene-transformed cells transform rat cells in cooperation with the ras oncogene and produce a protein that bound with the heat shock proteins. Recombinant clones produced between a Meth A cDNA and 11-4 were tested for the ability to transform rat cells. A single amino acid substitution at residue 132 was sufficientmore » to activate the 11-4 p53 cDNA for transformation. These studies have identified a region between amino acids 132 and 215 in the p53 protein which, when mutated, can activate the p53 cDNA. These results also call into question what the correct p53 wild-type sequence is and whether a wild-type p53 gene can transform cells in culture.« less

Authors:
; ; ; ; ;
Publication Date:
Research Org.:
Dept. of Molecular Biology, Princeton Univ., Princeton, NJ 08544 (US)
OSTI Identifier:
5141119
Resource Type:
Journal Article
Journal Name:
Mol. Cell. Biol.; (United States)
Additional Journal Information:
Journal Volume: 8:2
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; 3-METHYLCHOLANTHRENE; BIOLOGICAL EFFECTS; MUTAGENESIS; ONCOGENES; GENE AMPLIFICATION; MOLECULAR BIOLOGY; RECOMBINANT DNA; DNA-CLONING; AMINO ACIDS; CALMODULIN; CELL CULTURES; CELL TRANSFORMATIONS; DNA BASE TRANSITIONS; FIBROBLASTS; GENE RECOMBINATION PROTEINS; MONOCLONAL ANTIBODIES; MUTANTS; RATS; THERMAL SHOCK; ANIMAL CELLS; ANIMALS; ANTIBODIES; AROMATICS; CARBOXYLIC ACIDS; CLONING; CONNECTIVE TISSUE CELLS; DNA; GENES; HYDROCARBONS; MAMMALS; NUCLEIC ACIDS; NUCLEOPROTEINS; ORGANIC ACIDS; ORGANIC COMPOUNDS; POLYCYCLIC AROMATIC HYDROCARBONS; PROTEINS; RODENTS; SOMATIC CELLS; VERTEBRATES; 550400* - Genetics; 550200 - Biochemistry; 560300 - Chemicals Metabolism & Toxicology

Citation Formats

Finlay, C.A., Hinds, P.W., Tan, T.H., Eliyahu, D., Oren, M., and Levine, A.J. Activating mutations for transformation by p53 produce a gene product that forms an hsc70-p53 complex with an altered half-life. United States: N. p., 1988. Web. doi:10.1128/MCB.8.2.531.
Finlay, C.A., Hinds, P.W., Tan, T.H., Eliyahu, D., Oren, M., & Levine, A.J. Activating mutations for transformation by p53 produce a gene product that forms an hsc70-p53 complex with an altered half-life. United States. doi:10.1128/MCB.8.2.531.
Finlay, C.A., Hinds, P.W., Tan, T.H., Eliyahu, D., Oren, M., and Levine, A.J. Mon . "Activating mutations for transformation by p53 produce a gene product that forms an hsc70-p53 complex with an altered half-life". United States. doi:10.1128/MCB.8.2.531.
@article{osti_5141119,
title = {Activating mutations for transformation by p53 produce a gene product that forms an hsc70-p53 complex with an altered half-life},
author = {Finlay, C.A. and Hinds, P.W. and Tan, T.H. and Eliyahu, D. and Oren, M. and Levine, A.J.},
abstractNote = {The 11-4 p53 cDNA clone failed to transform primary rat fibroblasts when cotransfected with the ras oncogene. Two linker insertion mutations at amino acid 158 or 215 (of 390 amino acids) activated this p53 cDNA for transformation with ras. These mutant cDNAs produced a p53 protein that lacked an epitope, recognized by monoclonal antibody PAb246 (localized at amino acids 88 to 110 in the protein) and preferentially bound to a heat shock protein, hsc70. In rat cells transformed by a genomic p53 clone plus ras, two populations of p53 proteins were detected, PAb246/sup +/ and PAb246/sup -/, which did or did not bind to this monoclonal antibody, respectively. The PAb246/sup -/ p53 preferentially associated with hsc70, and this protein has a half-life 4- to 20-fold longer than free p53 (PAb246/sup +/). These data suggest a possible functional role for hsc70 in the transformation process. cDNAs for p53 derived from methylcholanthrene-transformed cells transform rat cells in cooperation with the ras oncogene and produce a protein that bound with the heat shock proteins. Recombinant clones produced between a Meth A cDNA and 11-4 were tested for the ability to transform rat cells. A single amino acid substitution at residue 132 was sufficient to activate the 11-4 p53 cDNA for transformation. These studies have identified a region between amino acids 132 and 215 in the p53 protein which, when mutated, can activate the p53 cDNA. These results also call into question what the correct p53 wild-type sequence is and whether a wild-type p53 gene can transform cells in culture.},
doi = {10.1128/MCB.8.2.531},
journal = {Mol. Cell. Biol.; (United States)},
number = ,
volume = 8:2,
place = {United States},
year = {1988},
month = {2}
}