Iron-mediated induction of the SOS responses by hydrogen peroxide

Journal Article · Mon Apr 01 00:00:00 EST 1991 · Bulletin of Environmental Contamination and Toxicology; (United States)

DOI:https://doi.org/10.1007/BF01688207· OSTI ID:5138545

Zhou, Renqing; Sun, Xinian; Tang, Datong ^[1]

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1. Liaoning Univ., Shenyang (China)

In Escherichia coli some of the responses induced by DNA-damaging agents involve a set of functions known as the SOS responses as well as damage-inducible genes. The SOS responses can be detected with the SOS chromotest in which the tester strain E. coli PQ37 is used. This strain carries a sfiA::lacZ fusion lacing lacZ, the structural gene for the beta-galactosidase, under control of the sfiA gene which is one of the damage-inducible genes, and has a deletion for normal lac region so that beta-galactosidase activity is strictly dependent on sfiA expression and can be taken as an indicator of DNA-damaging agents.

• OSTI ID: 5138545
• Journal Information: Bulletin of Environmental Contamination and Toxicology; (United States), Vol. 46:4; ISSN 0007-4861
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
DNA REPAIR
MOLECULAR BIOLOGY
SUPEROXIDE DISMUTASE
ENZYME INDUCTION
ENZYME ACTIVITY
ESCHERICHIA COLI
GALACTOSIDASE
BACTERIA
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
ENZYMES
GENE REGULATION
GLYCOSYL HYDROLASES
HYDROLASES
MICROORGANISMS
O-GLYCOSYL HYDROLASES
ORGANIC COMPOUNDS
OXIDOREDUCTASES
PROTEINS
REPAIR
560300* - Chemicals Metabolism & Toxicology