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Title: Enzymatic hydrolysis of cellulose to glucose using immobilized /beta/-glucosidase

Abstract

This research investigated the use of supplemental immobilized /beta/-glucosidase to increase yields of glucose. Immobilized /beta/-glucosidase from Aspergillus phoenicis was produced by sorption on controlled-pore alumina with about 90% activity retention. The product lost only about 10% of the original activity during an on-stream reaction period of 500 hr with cellobiose as substrate; maximum activity occurred near pH 3.5 and the apparent activation energy was about 11 kcal/mol. The immobilized /beta/-glucosidase was used together with Trichoderma reesei cellulase to hydrolyze cellulosic materials, such as Solka Floc, corn stover, and exploded wood. Increased yields of glucose and greater conversions of cellobiose to glucose were observed when the reaction systems contained supplemental immobilized /beta/-glucosidase.

Authors:
; ; ; ;
Publication Date:
Research Org.:
Univ. of Connecticut, Storrs
OSTI Identifier:
5136238
Resource Type:
Journal Article
Journal Name:
Biotechnol. Bioeng.; (United States)
Additional Journal Information:
Journal Volume: 23:3
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; CELLULOSE; ENZYMATIC HYDROLYSIS; GLUCOSE; PRODUCTION; BIOMASS; CELLOBIOSE; CELLULASE; GLUCOSIDASE; IMMOBILIZED ENZYMES; TRICHODERMA; ALDEHYDES; CARBOHYDRATES; CHEMICAL REACTIONS; DECOMPOSITION; DISACCHARIDES; ENERGY SOURCES; ENZYMES; FUNGI; GLYCOSYL HYDROLASES; HEXOSES; HYDROLASES; HYDROLYSIS; LYSIS; MONOSACCHARIDES; O-GLYCOSYL HYDROLASES; OLIGOSACCHARIDES; ORGANIC COMPOUNDS; PLANTS; POLYSACCHARIDES; RENEWABLE ENERGY SOURCES; SACCHARIDES; SOLVOLYSIS; 550200* - Biochemistry

Citation Formats

Sundstrom, D W, Klei, H E, Coughlin, R W, Biederman, G J, and Brouwer, C A. Enzymatic hydrolysis of cellulose to glucose using immobilized /beta/-glucosidase. United States: N. p., 1981. Web. doi:10.1002/bit.260230302.
Sundstrom, D W, Klei, H E, Coughlin, R W, Biederman, G J, & Brouwer, C A. Enzymatic hydrolysis of cellulose to glucose using immobilized /beta/-glucosidase. United States. https://doi.org/10.1002/bit.260230302
Sundstrom, D W, Klei, H E, Coughlin, R W, Biederman, G J, and Brouwer, C A. 1981. "Enzymatic hydrolysis of cellulose to glucose using immobilized /beta/-glucosidase". United States. https://doi.org/10.1002/bit.260230302.
@article{osti_5136238,
title = {Enzymatic hydrolysis of cellulose to glucose using immobilized /beta/-glucosidase},
author = {Sundstrom, D W and Klei, H E and Coughlin, R W and Biederman, G J and Brouwer, C A},
abstractNote = {This research investigated the use of supplemental immobilized /beta/-glucosidase to increase yields of glucose. Immobilized /beta/-glucosidase from Aspergillus phoenicis was produced by sorption on controlled-pore alumina with about 90% activity retention. The product lost only about 10% of the original activity during an on-stream reaction period of 500 hr with cellobiose as substrate; maximum activity occurred near pH 3.5 and the apparent activation energy was about 11 kcal/mol. The immobilized /beta/-glucosidase was used together with Trichoderma reesei cellulase to hydrolyze cellulosic materials, such as Solka Floc, corn stover, and exploded wood. Increased yields of glucose and greater conversions of cellobiose to glucose were observed when the reaction systems contained supplemental immobilized /beta/-glucosidase.},
doi = {10.1002/bit.260230302},
url = {https://www.osti.gov/biblio/5136238}, journal = {Biotechnol. Bioeng.; (United States)},
number = ,
volume = 23:3,
place = {United States},
year = {Sun Mar 01 00:00:00 EST 1981},
month = {Sun Mar 01 00:00:00 EST 1981}
}