Two-dimensional analysis of human lymphocyte proteins. III. Preliminary report on a marker for the early detection and diagnosis of infectious mononucleosis

Journal Article · Thu Apr 01 00:00:00 EST 1982 · Clin. Chem. (Winston-Salem, N.C.); (United States)

OSTI ID:5136143

Willard, K E

Two-dimensional gel electrophoretic patterns of human peripheral blood leukocytes from 12 patients with infectious mononucleosis were prepared by use of the ISO-DALT system. Before the two-dimensional separation, the leukocytes were purified by Ficoll-Paque gradient centrifugation and labeled overnight with (/sup 35/S) methionine. Quantitative increases in two proteins were detected in the patterns of infected leukocytes from the patients as compared with controls. Fluorescence-activated cell sorting of leukocytes from normal human peripheral blood before subsequent two-dimensional gel analysis revealed that the dramatic increase in one of these proteins (Inmono:2) could be due to shifts in the population ratios of lymphocytes, monocytes, and granulocytes. In contrast, the appearance in the infected leukocytes of a second protein, Inmono:1, could not be accounted for by cell-population shifts. Increased amounts of these two proteins have been found in every patient studied who had clinically detectable infectious mononucleosis. In addition, a patient who displayed symptoms of infectious mononucleosis but who did not have a positive result in the MONOSPOT test (Ortho) until three weeks after our analysis also demonstrated increased relative amounts of these proteins in his leukocyte pattern.

• Research Organization: Argonne National Lab., IL
• OSTI ID: 5136143
• Journal Information: Clin. Chem. (Winston-Salem, N.C.); (United States), Vol. 28:4
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
INFECTIOUS DISEASES
BIOLOGICAL INDICATORS
DIAGNOSIS
PROTEINS
BIOSYNTHESIS
ELECTROPHORESIS
AUTORADIOGRAPHY
FLUORESCENCE
HEMIC DISEASES
LABELLED COMPOUNDS
LYMPHOCYTES
MAN
METHIONINE
PATIENTS
SULFUR 35
AMINO ACIDS
ANIMAL CELLS
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CARBOXYLIC ACIDS
CONNECTIVE TISSUE CELLS
DAYS LIVING RADIOISOTOPES
DISEASES
DRUGS
EVEN-ODD NUCLEI
ISOTOPES
LEUKOCYTES
LIGHT NUCLEI
LIPOTROPIC FACTORS
LUMINESCENCE
MAMMALS
MATERIALS
NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
PRIMATES
RADIOISOTOPES
SOMATIC CELLS
SULFUR ISOTOPES
SYNTHESIS
VERTEBRATES
550201* - Biochemistry- Tracer Techniques