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Title: Nitrogen isotope ratio analysis of small samples: sample preparation and calibration

Abstract

A method for the preparation of samples in the range of 14-100 ..mu..g of organic and precipitated N for mass spectrometric isotope ratio analysis is described. In vacuo combustion and molecular sieve trapping of the sample gas into a 0.5 mL mass spectrometer inlet volume are employed. Samples can be processed at a rate of two to three per hour. The standard deviation of 12 analyses of a peptone preparation was 0.10%. The results of an interlaboratory calibration effort are presented. The materials tested were peptone, thiourea, histidine, kerogen, and two (NH/sub 4/)/sub 2/SO/sub 4/ standards. The results of these comparisons were with the range +/-0.12 delta/sup 15/N or less. Peptone was found to provide a more rigorous test of the combustion and preparation system than nitrogen salts and organic compounds of lower molecular weight. 20 references, 1 figure, 1 table.

Authors:
; ;
Publication Date:
Research Org.:
Harvard Univ., Cambridge, MA
OSTI Identifier:
5134948
Resource Type:
Journal Article
Journal Name:
Anal. Chem.; (United States)
Additional Journal Information:
Journal Volume: 57:11
Country of Publication:
United States
Language:
English
Subject:
37 INORGANIC, ORGANIC, PHYSICAL AND ANALYTICAL CHEMISTRY; AMMONIUM SULFATES; QUANTITATIVE CHEMICAL ANALYSIS; HISTIDINE; KEROGEN; NITROGEN; ISOTOPE RATIO; MASS SPECTROSCOPY; PEPTONE; THIOUREA; CALIBRATION STANDARDS; EXPERIMENTAL DATA; NITROGEN 15; SAMPLE PREPARATION; AMINO ACIDS; AMMONIUM COMPOUNDS; ANTITHYROID DRUGS; AZOLES; BITUMINOUS MATERIALS; CARBONACEOUS MATERIALS; CARBONIC ACID DERIVATIVES; CARBOXYLIC ACIDS; CHEMICAL ANALYSIS; DATA; DRUGS; ELEMENTS; HETEROCYCLIC ACIDS; HETEROCYCLIC COMPOUNDS; HORMONE ANTAGONISTS; IMIDAZOLES; INFORMATION; ISOTOPES; LIGHT NUCLEI; MATERIALS; NITROGEN ISOTOPES; NONMETALS; NUCLEI; NUMERICAL DATA; ODD-EVEN NUCLEI; ORGANIC ACIDS; ORGANIC COMPOUNDS; ORGANIC MATTER; ORGANIC NITROGEN COMPOUNDS; ORGANIC SULFUR COMPOUNDS; OXYGEN COMPOUNDS; PROTEINS; SPECTROSCOPY; STABLE ISOTOPES; STANDARDS; SULFATES; SULFUR COMPOUNDS; THIOUREAS; 400104* - Spectral Procedures- (-1987)

Citation Formats

Nevins, J L, Altabet, M A, and McCarthy, J J. Nitrogen isotope ratio analysis of small samples: sample preparation and calibration. United States: N. p., 1985. Web. doi:10.1021/ac00288a031.
Nevins, J L, Altabet, M A, & McCarthy, J J. Nitrogen isotope ratio analysis of small samples: sample preparation and calibration. United States. https://doi.org/10.1021/ac00288a031
Nevins, J L, Altabet, M A, and McCarthy, J J. 1985. "Nitrogen isotope ratio analysis of small samples: sample preparation and calibration". United States. https://doi.org/10.1021/ac00288a031.
@article{osti_5134948,
title = {Nitrogen isotope ratio analysis of small samples: sample preparation and calibration},
author = {Nevins, J L and Altabet, M A and McCarthy, J J},
abstractNote = {A method for the preparation of samples in the range of 14-100 ..mu..g of organic and precipitated N for mass spectrometric isotope ratio analysis is described. In vacuo combustion and molecular sieve trapping of the sample gas into a 0.5 mL mass spectrometer inlet volume are employed. Samples can be processed at a rate of two to three per hour. The standard deviation of 12 analyses of a peptone preparation was 0.10%. The results of an interlaboratory calibration effort are presented. The materials tested were peptone, thiourea, histidine, kerogen, and two (NH/sub 4/)/sub 2/SO/sub 4/ standards. The results of these comparisons were with the range +/-0.12 delta/sup 15/N or less. Peptone was found to provide a more rigorous test of the combustion and preparation system than nitrogen salts and organic compounds of lower molecular weight. 20 references, 1 figure, 1 table.},
doi = {10.1021/ac00288a031},
url = {https://www.osti.gov/biblio/5134948}, journal = {Anal. Chem.; (United States)},
number = ,
volume = 57:11,
place = {United States},
year = {Sun Sep 01 00:00:00 EDT 1985},
month = {Sun Sep 01 00:00:00 EDT 1985}
}