Role of calcium in the mechanical strength of soybean hypocotyl cell walls
Abstract
Calcium ions inhibit auxin-induced growth in both dicot stems and coleoptiles. In coleoptiles calcium does not directly stiffen cell walls. The authors have tested here whether calcium might alter the mechanical strength of a dicot cell wall, the soybean hypocotyl. Sections were longitudinally bisected, boiled or frozen-thawed, incubated in solutions and then the mechanical strength was determined with an Instron. The calcium content was also measured. Removal of calcium by EGTA or by acidic buffers such as K-Pi-citrate resulted in a proportional increase in wall extensibility. Addition of calcium, on the other hand, stiffened the walls. These changes were reversible. It was concluded that calcium crosslinks make a significant contribution to the strength of dicot stem cell walls, and that in vivo, removal of calcium from the wall by uptake into the cell could result in wall loosening and thus enhanced growth.
- Authors:
- Publication Date:
- Research Org.:
- Univ. of Washington, Seattle
- OSTI Identifier:
- 5127720
- DOE Contract Number:
- AT06-70ER73019
- Resource Type:
- Conference
- Journal Name:
- Plant Physiol., Suppl.; (United States)
- Additional Journal Information:
- Journal Volume: 80:4; Conference: Annual meeting of the American Society of Plant Physiologists, Baton Rouge, LA, USA, 8-12 Jun 1986
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; CALCIUM; BIOLOGICAL EFFECTS; CELL WALL; ELASTICITY; PLANT GROWTH; INHIBITION; AUXINS; PHASEOLUS; ALKALINE EARTH METALS; BACTERIA; CELL CONSTITUENTS; ELEMENTS; GROWTH; LEGUMINOSAE; MECHANICAL PROPERTIES; METALS; MICROORGANISMS; PLANT GROWTH REGULATORS; PLANTS; RHIZOBIUM; TENSILE PROPERTIES; 550200* - Biochemistry
Citation Formats
Virk, S S, and Cleland, R E. Role of calcium in the mechanical strength of soybean hypocotyl cell walls. United States: N. p., 1986.
Web.
Virk, S S, & Cleland, R E. Role of calcium in the mechanical strength of soybean hypocotyl cell walls. United States.
Virk, S S, and Cleland, R E. 1986.
"Role of calcium in the mechanical strength of soybean hypocotyl cell walls". United States.
@article{osti_5127720,
title = {Role of calcium in the mechanical strength of soybean hypocotyl cell walls},
author = {Virk, S S and Cleland, R E},
abstractNote = {Calcium ions inhibit auxin-induced growth in both dicot stems and coleoptiles. In coleoptiles calcium does not directly stiffen cell walls. The authors have tested here whether calcium might alter the mechanical strength of a dicot cell wall, the soybean hypocotyl. Sections were longitudinally bisected, boiled or frozen-thawed, incubated in solutions and then the mechanical strength was determined with an Instron. The calcium content was also measured. Removal of calcium by EGTA or by acidic buffers such as K-Pi-citrate resulted in a proportional increase in wall extensibility. Addition of calcium, on the other hand, stiffened the walls. These changes were reversible. It was concluded that calcium crosslinks make a significant contribution to the strength of dicot stem cell walls, and that in vivo, removal of calcium from the wall by uptake into the cell could result in wall loosening and thus enhanced growth.},
doi = {},
url = {https://www.osti.gov/biblio/5127720},
journal = {Plant Physiol., Suppl.; (United States)},
number = ,
volume = 80:4,
place = {United States},
year = {Tue Apr 01 00:00:00 EST 1986},
month = {Tue Apr 01 00:00:00 EST 1986}
}