DNA sequence recognition protein associated with a multiprotein form of DNA polymerase alpha

Journal Article · Thu May 01 00:00:00 EDT 1986 · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)

OSTI ID:5127415

Malkas, L H; Baril, E F

The majority of DNA polymerase ..cap alpha.. activity in HeLa cells has been isolated and purified as a multiprotein Mr 640,000 form. A number of accessory activities cofractionate with this form of polymerase ..cap alpha... Among these are: Cl, C2 primer recognition proteins, primase, a 5' ..-->.. 3' exonuclease, and a 5',5''',P/sup 1/,P/sup 4/-diadenosine tetraphosphate (Ap/sub 4/A) binding protein. Preliminary results suggest an additional factor(s) or protein(s) is present in the multiprotein form of the HeLa cell DNA polymerase ..cap alpha.. which has an affinity for DNA sequences rich in A and T residues. Affinity chromatography on poly(dA)-or oligo(dT)-cellulose yields a highly purified protein. This protein has the ability to bind /sup 3/H-poly (dA) and /sup 3/H-poly(dT) in a nitrocellulose filter binding assay. The further physical properties of this protein and its DNA sequence binding specificity will be discussed.

• Research Organization: Worcester Foundation for Experimental Biology, Shrewsbury, MA
• OSTI ID: 5127415
• Report Number(s): CONF-8606151-; TRN: 86-034800
• Journal Information: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Vol. 45:6; Conference: 76. annual meeting of the Federation of American Society for Experimental Biology, Washington, DC, USA, 8 Jun 1986
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
DNA POLYMERASES
ENZYME ACTIVITY
PROTEINS
DNA SEQUENCING
PURIFICATION
AFFINITY
CHROMATOGRAPHY
HELA CELLS
PHYSICAL PROPERTIES
TRACER TECHNIQUES
TRITIUM COMPOUNDS
ENZYMES
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
NUCLEOTIDYLTRANSFERASES
ORGANIC COMPOUNDS
PHOSPHORUS-GROUP TRANSFERASES
POLYMERASES
SEPARATION PROCESSES
STRUCTURAL CHEMICAL ANALYSIS
TRANSFERASES
550201* - Biochemistry- Tracer Techniques