In vitro protein synthesis by plastids of Phaseolus vulgaris. I. Localization of activity in the chloroplasts of a chloroplast containing fraction from developing leaves

Journal Article · Sun Jan 01 00:00:00 EST 1967 · Plant Physiol.; (United States)

DOI:https://doi.org/10.1104/pp.42.9.1179· OSTI ID:5126961

Parenti, F; Margulies, M M

The incorporation of uniformly labeled leucine-/sup 14/C into protein by a chloroplast-containing fraction from developing primary leaves of bean is reported. Chloroplasts, obtained from week old plants grown in darkness, and then illuminated with white light for 12 hours, were shown to be the principal sites of incorporating activity. Incorporation may continue for 2 hours. Rates of up to 50 ..mu mu..mole leucine incorporated per mg protein per hour are observed when a 1 hour assay period is used. Incorporation is only partially sensitive to ribonuclease. 33 references, 3 figures, 3 tables.

• Research Organization: Smithsonian Institution, Washington, DC
• OSTI ID: 5126961
• Journal Information: Plant Physiol.; (United States), Vol. 42
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
PROTEINS
BIOSYNTHESIS
CARBON 14
CHLOROPLASTS
LEAVES
LEUCINE
NUCLEASES
PHASEOLUS
TRACER TECHNIQUES
AMINO ACIDS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CARBON ISOTOPES
CARBOXYLIC ACIDS
CELL CONSTITUENTS
ENZYMES
ESTERASES
EVEN-EVEN NUCLEI
HYDROLASES
ISOTOPE APPLICATIONS
ISOTOPES
LEGUMINOSAE
LIGHT NUCLEI
NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
PHOSPHODIESTERASES
PLANTS
RADIOISOTOPES
SYNTHESIS
YEARS LIVING RADIOISOTOPES
550501* - Metabolism- Tracer Techniques