Radiation resistance of lactobacilli isolated from radurized meat relative to growth and environment. [Lactobacillus sake; Lactobacillus curvatus; Lactobacillus farciminis; Staphylococcus aureus; Salmonella typimurium]

Journal Article · Wed Oct 01 00:00:00 EDT 1986 · Appl. Environ. Microbiol.; (United States)

OSTI ID:5118745

Hastings, J W; Holzapfel, W H; Niemand, J G

Of 113 lactobacilli isolated from radurized (5 kGy) minced meat, 7 Lactobacillus sake strains, 1 L. curvatus strain, and 1 L. farciminis strain were used for radiation resistance studies in a semisynthetic substrate (i.e., modified MRS broth). Five reference Lactobacillus spp. one Staphylococcus aureus strain, and one Salmonella typhimurium strain were used for comparative purposes. All L. sake isolates exhibited the phenomenon of being more resistant to gamma-irradiation in the exponential (log) phase than in the stationary phase of their growth cycles by a factor of 28%. Four reference strains also exhibited this phenomenon, with L. sake (DSM 20017) showing a 68% increase in resistance in the log phase over the stationary phase. This phenomenon was not common to all bacteria tested and is not common to all strains with high radiation resistance. Four L. sake isolates and three reference strains were used in radiation sensitivity testing in a natural food system (i.e., meat). The bacteria were irradiated in minced meat and packaged under four different conditions (air, vacuum, CO/sub 2/, and N/sub 2/). Organisms exhibited the highest death rate (lowest D/sub 10/ values (doses required to reduce the logarithm of the bacterial population by 1) under CO/sub 2/ packaging conditions, but resistance to irradiation was increased under N/sub 2/. The D/sup 10/ values of the isolates were generally greater than those of the reference strains. The D/sup 10/ values were also higher (approximately two times) in meat than in a semisynthetic growth medium.

• Research Organization: Atomic Energy Corp., Pretoria, South Africa
• OSTI ID: 5118745
• Journal Information: Appl. Environ. Microbiol.; (United States), Vol. 52:4
• Country of Publication: United States
• Language: English

Related Subjects

60 APPLIED LIFE SCIENCES
LACTOBACILLUS
RADIOSENSITIVITY
MEAT
PACKAGING
RADURIZATION
AIR
CARBON DIOXIDE
EXPERIMENTAL DATA
GAMMA RADIATION
NITROGEN
VIABILITY
BACTERIA
CARBON COMPOUNDS
CARBON OXIDES
CHALCOGENIDES
DATA
ELECTROMAGNETIC RADIATION
ELEMENTS
FLUIDS
FOOD
GASES
INFORMATION
IONIZING RADIATIONS
MICROORGANISMS
NONMETALS
NUMERICAL DATA
OXIDES
OXYGEN COMPOUNDS
RADIATIONS
070202* - Radiation Sources- Use in Food Processing- (-1987)