Stable variant-specific transcripts of the variant cell surface glycoprotein gene 1. 8 expression site in Trypanosoma brucei
The structure and transcriptional regulation of the 1.8 variant cell surface glycoproteins (VSG) gene expression site located on a 430-kilobase (kb) chromosome was examined in a 430-kb-chromosome-specific library. Using /sup 32/P-labeled nascent transcripts generated by nuclear run-on, the authors selected recombinant clones derived from the 430-kb chromosome which were coordinately activated with the 1.8 VSG gene. The results show that a repetitive region with a minimum size of 27 kb is coordinately activated with the 1.8 VSG gene. As with the 1.8 VSG gene, transcription is by RNA polymerases that are insensitive to the drug alpha-amanitin at concentrations up to 1 mgml. Transcription results in the generation of several stable variant-specific mRNAs. These mRNAs most likely belong to a family of repetitive expression-site-associated genes.
- Research Organization:
- Dept. of Genetics and Development, Columbia Univ., New York, NY 10032 (US)
- OSTI ID:
- 5108963
- Journal Information:
- Mol. Cell. Biol.; (United States), Vol. 8:2
- Country of Publication:
- United States
- Language:
- English
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GLUCOPROTEINS
GENES
MUTANTS
MESSENGER-RNA
TRYPANOSOMA
CHROMOSOMES
SURFACE PROPERTIES
CELL MEMBRANES
LABELLED COMPOUNDS
LABELLING
PHENOTYPE
PHOSPHORUS 32
RECOMBINANT DNA
RNA POLYMERASES
TRANSCRIPTION
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CARBOHYDRATES
CELL CONSTITUENTS
DAYS LIVING RADIOISOTOPES
DNA
ENZYMES
ISOTOPES
LIGHT NUCLEI
MASTIGOPHORA
MEMBRANES
NUCLEI
NUCLEIC ACIDS
NUCLEOTIDYLTRANSFERASES
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
PARASITES
PHOSPHORUS ISOTOPES
PHOSPHORUS-GROUP TRANSFERASES
POLYMERASES
PROTEINS
RADIOISOTOPES
RNA
SACCHARIDES
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550401* - Genetics- Tracer Techniques
550200 - Biochemistry