Betaine aldehyde dehydrogenase isozymes of spinach
Betaine is synthesized in spinach chloroplasts via the pathway Choline ..-->.. Betaine Aldehyde ..-->.. Betaine; the second step is catalyzed by betaine aldehyde dehydrogenase (BADH). The subcellular distribution of BADH was determined in leaf protoplast lysates; BADH isozymes were separated by 6-9% native PAGE. The chloroplast stromal fraction contains a single BADH isozyme (number1) that accounts for > 80% of the total protoplast activity; the extrachloroplastic fraction has a minor isozyme (number2) which migrates more slowly than number1. Both isozymes appear specific for betaine aldehyde, are more active with NAD than NADP, and show a ca. 3-fold activity increase in salinized leaves. The phenotype of a natural variant of isozyme number1 suggests that the enzyme is a dimer.
- Research Organization:
- Michigan State Univ., East Lansing
- DOE Contract Number:
- AC02-76ER01338
- OSTI ID:
- 5098189
- Journal Information:
- Plant Physiol., Suppl.; (United States), Vol. 80:4; Conference: Annual meeting of the American Society of Plant Physiologists, Baton Rouge, LA, USA, 8-12 Jun 1986
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
BETAINE
BIOSYNTHESIS
LEAVES
ENZYME ACTIVITY
BIOLOGICAL PATHWAYS
ENZYMES
NAD
NADP
SPINACH
AMINES
AMINO ACIDS
AMMONIUM COMPOUNDS
CARBOXYLIC ACIDS
COENZYMES
DRUGS
FOOD
LIPOTROPIC FACTORS
NUCLEOTIDES
ORGANIC ACIDS
ORGANIC COMPOUNDS
PLANTS
QUATERNARY COMPOUNDS
SYNTHESIS
VEGETABLES
550200* - Biochemistry