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Title: Relative importance of the bone marrow and spleen in the production and dissemination of B lymphocytes

Abstract

The relative importance of the bone marrow and spleen in the production of B lymphocytes was investigated in guinea pigs by the combined use of (/sup 3/H)TdR radioautography and fluorescent microscopy after the staining of B cells by FITC-F (ab')/sub 2/-goat-anti-guinea pig Ig. Large and small lymphoid cells possess sIg in the marrow and spleen but B cell turnover in the marrow exceeds that in the spleen. That newly generated bone marrow B cells are not derived from an extramyeloid bursa equivalent was demonstrated by the absence of (/sup 3/H)TdR labeled B cells in tibial marrow 72 hr after (/sup 3/H)TdR was administered systemically, while the circulation to the hind limbs was occluded. Pulse and chase studies with (/sup 3/H)TdR showed that large marrow B cells are derived from sIg-negative, proliferating precursors resident in the bone marrow and not from the enlargement of activated small B lymphocytes. The acquisition of (/sup 3/H)TdR by splenic B cells lagged behind that observed in the marrow. Three days after topical labeling of tibial and femoral bone marrow with (/sup 3/H)TdR, a substantial proportion of splenic B cells were replaced by cells that had seeded there from the labeled marrow. The studies unequivocally identifymore » the bone marrow as the organ of primary importance in B cell generation, and indicate that in the guinea pig rapidly renewed B lymphocytes of the spleen are replaced by lymphocytes recently generated in bone marrow. The rate of replacement of B lymphocytes in the lymph node by cells newly generated in the bone marrow takes place at a slower tempo than in the spleen.« less

Authors:
; ; ; ;
Publication Date:
Research Org.:
Univ. of Washington, Seattle
OSTI Identifier:
5090492
Resource Type:
Journal Article
Journal Name:
Cell. Immunol.; (United States)
Additional Journal Information:
Journal Volume: 37:1
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; BONE MARROW; LEUKOPOIESIS; SPLEEN; AUTORADIOGRAPHY; COMPARATIVE EVALUATIONS; GUINEA PIGS; LYMPHOCYTES; TRITIUM COMPOUNDS; ANIMAL CELLS; ANIMALS; BIOLOGICAL MATERIALS; BLOOD; BLOOD CELLS; BLOOD FORMATION; BODY; BODY FLUIDS; CONNECTIVE TISSUE CELLS; HEMATOPOIETIC SYSTEM; LABELLED COMPOUNDS; LEUKOCYTES; MAMMALS; ORGANS; RODENTS; SOMATIC CELLS; TISSUES; VERTEBRATES; 551001* - Physiological Systems- Tracer Techniques; 550301 - Cytology- Tracer Techniques

Citation Formats

Rosse, C., Cole, S.B., Appleton, C., Press, O.W., and Clagett, J. Relative importance of the bone marrow and spleen in the production and dissemination of B lymphocytes. United States: N. p., 1978. Web. doi:10.1016/0008-8749(78)90192-2.
Rosse, C., Cole, S.B., Appleton, C., Press, O.W., & Clagett, J. Relative importance of the bone marrow and spleen in the production and dissemination of B lymphocytes. United States. doi:10.1016/0008-8749(78)90192-2.
Rosse, C., Cole, S.B., Appleton, C., Press, O.W., and Clagett, J. Sat . "Relative importance of the bone marrow and spleen in the production and dissemination of B lymphocytes". United States. doi:10.1016/0008-8749(78)90192-2.
@article{osti_5090492,
title = {Relative importance of the bone marrow and spleen in the production and dissemination of B lymphocytes},
author = {Rosse, C. and Cole, S.B. and Appleton, C. and Press, O.W. and Clagett, J.},
abstractNote = {The relative importance of the bone marrow and spleen in the production of B lymphocytes was investigated in guinea pigs by the combined use of (/sup 3/H)TdR radioautography and fluorescent microscopy after the staining of B cells by FITC-F (ab')/sub 2/-goat-anti-guinea pig Ig. Large and small lymphoid cells possess sIg in the marrow and spleen but B cell turnover in the marrow exceeds that in the spleen. That newly generated bone marrow B cells are not derived from an extramyeloid bursa equivalent was demonstrated by the absence of (/sup 3/H)TdR labeled B cells in tibial marrow 72 hr after (/sup 3/H)TdR was administered systemically, while the circulation to the hind limbs was occluded. Pulse and chase studies with (/sup 3/H)TdR showed that large marrow B cells are derived from sIg-negative, proliferating precursors resident in the bone marrow and not from the enlargement of activated small B lymphocytes. The acquisition of (/sup 3/H)TdR by splenic B cells lagged behind that observed in the marrow. Three days after topical labeling of tibial and femoral bone marrow with (/sup 3/H)TdR, a substantial proportion of splenic B cells were replaced by cells that had seeded there from the labeled marrow. The studies unequivocally identify the bone marrow as the organ of primary importance in B cell generation, and indicate that in the guinea pig rapidly renewed B lymphocytes of the spleen are replaced by lymphocytes recently generated in bone marrow. The rate of replacement of B lymphocytes in the lymph node by cells newly generated in the bone marrow takes place at a slower tempo than in the spleen.},
doi = {10.1016/0008-8749(78)90192-2},
journal = {Cell. Immunol.; (United States)},
number = ,
volume = 37:1,
place = {United States},
year = {1978},
month = {4}
}