Dominant lethal assay for bis-dinitro-propyl-formal: bis-dinitro-propyl-acetal and kerimide 601 resin

Technical Report · Wed Mar 01 00:00:00 EST 1978

DOI:https://doi.org/10.2172/5087153· OSTI ID:5087153

Wilson, J S; Smith, D M

Bis-dinitro-propyl-formal:bis-dinitro-propyl-acetal (BDNPF:BDNPA) and kerimide 601 resin do not appear to be mutagenic at the doses utilized when introduced intragastrically either as an acute or chronic insult in vivo. It should be pointed out that the dominant lethal assay has limitations. It has been observed by others that positive data with Ames testing can give negative data with this method. To produce a strong mutagenic effect detectable by the dominant lethal assay, there must be considerable chromosome damage in the sperm. The dominant lethal assay does not detect point mutations or low incidence of chromosome breakage. The assay can be valuable in expressing the in vivo effect, which can be contrary to in vitro studies, and should be considered in a total toxicity survey of a chemical. Using the dominant lethal assay, one can screen out a positive mutagen, but lack of an effect does not indicate that the chemical is not mutagenic. It is either not mutagenic or the assay is not specific enough to detect the in vivo effect.

• Research Organization: Los Alamos National Lab. (LANL), Los Alamos, NM (United States)
• DOE Contract Number: W-7405-ENG-36
• OSTI ID: 5087153
• Report Number(s): LA-7208-MS
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
59 BASIC BIOLOGICAL SCIENCES
DOMINANT MUTATIONS
BIOASSAY
LETHAL MUTATIONS
RESINS
GENETIC EFFECTS
CHROMOSOMES
MUTAGENESIS
SPERMATOZOA
TOXICITY
BIOLOGICAL EFFECTS
GAMETES
GERM CELLS
MUTATIONS
ORGANIC COMPOUNDS
ORGANIC POLYMERS
PETROCHEMICALS
PETROLEUM PRODUCTS
POLYMERS
560305* - Chemicals Metabolism & Toxicology- Vertebrates- (-1987)
550400 - Genetics