Expression of lip genes during growth in soil and oxidation of anthracene by Phanerochaete chrysosporium
- Univ. of Wisconsin, Madison, WI (United States)
- Inst. for Microbial and Biochemical Technology, Madison, WI (United States)
mRNA extraction from soil and quantitation by competitive reverse transcription-PCR were combined to study the expression of the 10 known lignin peroxidase (lip) genes in anthracene-transforming soil cultures of Phanerochaete chrysosporium. Levels of extractable lipA transcript and protein (LiP H8) were well correlated, although they were separated by a 2-day lag period. The patterns of transcript abundance over time in soil-grown P. chrysosporium varied among the nine lip mRNAs detected; comparison with lip gene expression under different liquid culture conditions suggested an early phase of carbon limitation for the cultures as a whole, which was followed by a transition to nitrogen starvation. Anthracene transformation occurred through-out the 25-day course of the experiment and, therefore, likely involves mechanisms distinct from those involved in oxidation of non-LiP substrate polycyclic aromatic hydrocarbons. 54 refs., 6 figs.
- DOE Contract Number:
- FG02-87ER13712
- OSTI ID:
- 508350
- Journal Information:
- Applied and Environmental Microbiology, Vol. 62, Issue 10; Other Information: PBD: Oct 1996
- Country of Publication:
- United States
- Language:
- English
Similar Records
Homologous expression of recombinant lignin peroxidase in Phanerochaete chrysosporium
Quantitation of fungal mRNAs in complex substrates by reverse transciption pcr and its application to Phanerochaete chrysosporium-colonized soil