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Title: Carcinoembryonic antigen: assay following heat compared with perchloric acid extraction in patients with colon cancer, non-neoplastic gastrointestinal diseases, or chronic renal failure

Abstract

Heat inactivation has been proposed as an alternative to perchloric acid (PCA) precipitation for the extraction of carcinoembryonic antigen (CEA) from human plasma. A commercial RIA kit using heat inactivation was examined and results compared with those obtained with PCA precipitation. Adequate sensitivity (1.5 ..mu..g CEA/I plasma), satisfactory analytical recovery of CEA added to plasma, and dilutional linearity of samples found to have elevated CEA concentrations, were demonstrated for the heat-inactivation assay. Between-assay precision was better with the heat inactivation than with the PCA assay. Although the absolute concentration of CEA estimated after heat inactivation was consistently lower than that estimated after PCA extraction of plasma specimens, there was excellent correlation between results obtained with the two methods in colon cancer patients free of disease, colon cancer patients with residual or recurrent disease, patients with benign gastrointestinal disease, and in patients with chronic renal failure. The heat-inactivation assay is an excellent alternative to the PCA assay.

Authors:
; ; ;
Publication Date:
Research Org.:
Ochsner Clinic; Alton Ochsner Medical Foundation, New Orleans, LO
OSTI Identifier:
5076808
Resource Type:
Journal Article
Journal Name:
J. Nucl. Med.; (United States)
Additional Journal Information:
Journal Volume: 24:10
Country of Publication:
United States
Language:
English
Subject:
62 RADIOLOGY AND NUCLEAR MEDICINE; CARCINOEMBRYONIC ANTIGEN; CALIBRATION STANDARDS; RADIOIMMUNOASSAY; HEAT; COMPARATIVE EVALUATIONS; PERCHLORIC ACID; DIGESTIVE SYSTEM DISEASES; EXTRACTION; INACTIVATION; KIDNEYS; LARGE INTESTINE; NEOPLASMS; PATIENTS; ANTIGENS; BODY; DIGESTIVE SYSTEM; DISEASES; ENERGY; GASTROINTESTINAL TRACT; HYDROGEN COMPOUNDS; INORGANIC ACIDS; INTESTINES; ISOTOPE APPLICATIONS; ORGANS; RADIOASSAY; SEPARATION PROCESSES; STANDARDS; TRACER TECHNIQUES; 550601* - Medicine- Unsealed Radionuclides in Diagnostics

Citation Formats

Witherspoon, L R, Shuler, S E, Alyea, K, and Husserl, F E. Carcinoembryonic antigen: assay following heat compared with perchloric acid extraction in patients with colon cancer, non-neoplastic gastrointestinal diseases, or chronic renal failure. United States: N. p., 1983. Web.
Witherspoon, L R, Shuler, S E, Alyea, K, & Husserl, F E. Carcinoembryonic antigen: assay following heat compared with perchloric acid extraction in patients with colon cancer, non-neoplastic gastrointestinal diseases, or chronic renal failure. United States.
Witherspoon, L R, Shuler, S E, Alyea, K, and Husserl, F E. 1983. "Carcinoembryonic antigen: assay following heat compared with perchloric acid extraction in patients with colon cancer, non-neoplastic gastrointestinal diseases, or chronic renal failure". United States.
@article{osti_5076808,
title = {Carcinoembryonic antigen: assay following heat compared with perchloric acid extraction in patients with colon cancer, non-neoplastic gastrointestinal diseases, or chronic renal failure},
author = {Witherspoon, L R and Shuler, S E and Alyea, K and Husserl, F E},
abstractNote = {Heat inactivation has been proposed as an alternative to perchloric acid (PCA) precipitation for the extraction of carcinoembryonic antigen (CEA) from human plasma. A commercial RIA kit using heat inactivation was examined and results compared with those obtained with PCA precipitation. Adequate sensitivity (1.5 ..mu..g CEA/I plasma), satisfactory analytical recovery of CEA added to plasma, and dilutional linearity of samples found to have elevated CEA concentrations, were demonstrated for the heat-inactivation assay. Between-assay precision was better with the heat inactivation than with the PCA assay. Although the absolute concentration of CEA estimated after heat inactivation was consistently lower than that estimated after PCA extraction of plasma specimens, there was excellent correlation between results obtained with the two methods in colon cancer patients free of disease, colon cancer patients with residual or recurrent disease, patients with benign gastrointestinal disease, and in patients with chronic renal failure. The heat-inactivation assay is an excellent alternative to the PCA assay.},
doi = {},
url = {https://www.osti.gov/biblio/5076808}, journal = {J. Nucl. Med.; (United States)},
number = ,
volume = 24:10,
place = {United States},
year = {Sat Oct 01 00:00:00 EDT 1983},
month = {Sat Oct 01 00:00:00 EDT 1983}
}