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Title: Changes in the antigenic and molecular structure of. gamma. -irradiated bacterial lipopolysaccharide (LPS)

Abstract

Ionization radiation is known to alter the biological properties of LPS. The author treated a highly purified LPS from E. coli in aqueous medium with /sup 60/Co-radiation. The changes in the antigenic and molecular structure of LPS were studied in double immunodiffusion/immunoelectrophoresis (rabbit antiserum) and sodium dodecylsulfate-polyacrylamide gel electrophoresis (SDS-PAGE), respectively. Untreated LPS revealed two major antigenic components and, due to varying lengths of the O-polysaccharide side chain, a series of homopolymers (SDS-PAGE). High doses of ..gamma..-radiation destroyed all antigenic reactivities and all stainable bands on SDS-PAGE. However, lower doses of radiation were selective. Disappearance of the more radiation-sensitive, electrophoretically fast-migrating antigenic component paralleled elimination of the long O-side chain containing molecules. The relatively radiation-resistant, less anodic second antigenic component cross-reacted with LPS of another E. coli strain and corresponded to LPS molecules composed of R-core and lipid A (SDS-PAGE). These findings explain the in vivo loss of antibody protection from shock before non-specific resistance with ..gamma..-irradiation of LPS.

Authors:
; ; ;
Publication Date:
Research Org.:
National Institute of Health, Bethesda, MD
OSTI Identifier:
5072249
Report Number(s):
CONF-8604222-
Journal ID: CODEN: FEPRA; TRN: 86-037067
Resource Type:
Conference
Journal Name:
Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)
Additional Journal Information:
Journal Volume: 45:3; Conference: 70. annual meeting of the Federation of American Society for Experimental Biology, St. Louis, MO, USA, 13 Apr 1986
Country of Publication:
United States
Language:
English
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; LIPOPOLYSACCHARIDES; BIOLOGICAL RADIATION EFFECTS; MOLECULAR STRUCTURE; ANTIGENS; COBALT 60; ELECTROPHORESIS; ESCHERICHIA COLI; GAMMA RADIATION; IMMUNE REACTIONS; MOLECULES; BACTERIA; BETA DECAY RADIOISOTOPES; BETA-MINUS DECAY RADIOISOTOPES; BIOLOGICAL EFFECTS; CARBOHYDRATES; COBALT ISOTOPES; ELECTROMAGNETIC RADIATION; INTERMEDIATE MASS NUCLEI; INTERNAL CONVERSION RADIOISOTOPES; IONIZING RADIATIONS; ISOMERIC TRANSITION ISOTOPES; ISOTOPES; LIPIDS; MICROORGANISMS; MINUTES LIVING RADIOISOTOPES; NUCLEI; ODD-ODD NUCLEI; ORGANIC COMPOUNDS; POLYSACCHARIDES; RADIATION EFFECTS; RADIATIONS; RADIOISOTOPES; SACCHARIDES; YEARS LIVING RADIOISOTOPES; 560111* - Radiation Effects on Biochemicals- In Vitro- (-1987)

Citation Formats

Csako, G, Suba, E A, Tsai, C M, and Elin, R J. Changes in the antigenic and molecular structure of. gamma. -irradiated bacterial lipopolysaccharide (LPS). United States: N. p., 1986. Web.
Csako, G, Suba, E A, Tsai, C M, & Elin, R J. Changes in the antigenic and molecular structure of. gamma. -irradiated bacterial lipopolysaccharide (LPS). United States.
Csako, G, Suba, E A, Tsai, C M, and Elin, R J. Sat . "Changes in the antigenic and molecular structure of. gamma. -irradiated bacterial lipopolysaccharide (LPS)". United States.
@article{osti_5072249,
title = {Changes in the antigenic and molecular structure of. gamma. -irradiated bacterial lipopolysaccharide (LPS)},
author = {Csako, G and Suba, E A and Tsai, C M and Elin, R J},
abstractNote = {Ionization radiation is known to alter the biological properties of LPS. The author treated a highly purified LPS from E. coli in aqueous medium with /sup 60/Co-radiation. The changes in the antigenic and molecular structure of LPS were studied in double immunodiffusion/immunoelectrophoresis (rabbit antiserum) and sodium dodecylsulfate-polyacrylamide gel electrophoresis (SDS-PAGE), respectively. Untreated LPS revealed two major antigenic components and, due to varying lengths of the O-polysaccharide side chain, a series of homopolymers (SDS-PAGE). High doses of ..gamma..-radiation destroyed all antigenic reactivities and all stainable bands on SDS-PAGE. However, lower doses of radiation were selective. Disappearance of the more radiation-sensitive, electrophoretically fast-migrating antigenic component paralleled elimination of the long O-side chain containing molecules. The relatively radiation-resistant, less anodic second antigenic component cross-reacted with LPS of another E. coli strain and corresponded to LPS molecules composed of R-core and lipid A (SDS-PAGE). These findings explain the in vivo loss of antibody protection from shock before non-specific resistance with ..gamma..-irradiation of LPS.},
doi = {},
journal = {Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)},
number = ,
volume = 45:3,
place = {United States},
year = {1986},
month = {3}
}

Conference:
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