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Title: Measurements and models of synchronous growth of fission yeast induced by temperature oscillations. [Schizosaccharomyces pombe]

Abstract

Pulsing of temperature in a fermentor at intervals coincident with cell generation time was used to induce synchrony in a population of the fission yeast Schizosaccharomyces pombe. Measurements of culture protein, RNA, and DNA during synchronous growth confirm continuous synthesis of protein and RNA and discontinuous synthesis of DNA as previously reported. Flow microfluorometry of populations at different times during the synchrony cycle was used to monitor the changes in single-cell protein, RNA, and DNA frequency functions. These measurements illustrate very clearly the degree of synchrony and patterns of macromolecular synthesis and also confirm previous estimates of the cellular protein contents characteristic of dividing cells. Additional insights into single-cell kinetics and division controls are provided by two-parameter flow microfluorometry measurements and by mathematical modeling of population dynamics. Such data are necessary foundations for robust population balance models of microbial processes. (Refs. 31).

Authors:
;
Publication Date:
Research Org.:
Dept of Chemical Engineering, Univ of Houston, Houston, TX 77004
OSTI Identifier:
5049033
Resource Type:
Journal Article
Resource Relation:
Journal Name: Biotechnol. Bioeng.; (United States); Journal Volume: 24:1
Country of Publication:
United States
Language:
English
Subject:
09 BIOMASS FUELS; 59 BASIC BIOLOGICAL SCIENCES; SYNCHRONOUS CULTURES; KINETICS; MATHEMATICAL MODELS; YEASTS; DNA; GROWTH; RNA; SACCHAROMYCES; SINGLE CELL PROTEIN; TEMPERATURE EFFECTS; CELL CULTURES; FUNGI; MICROORGANISMS; NUCLEIC ACIDS; ORGANIC COMPOUNDS; PLANTS; 140504* - Solar Energy Conversion- Biomass Production & Conversion- (-1989); 550700 - Microbiology

Citation Formats

Agar, D.W., and Bailey, J.E. Measurements and models of synchronous growth of fission yeast induced by temperature oscillations. [Schizosaccharomyces pombe]. United States: N. p., 1982. Web. doi:10.1002/bit.260240118.
Agar, D.W., & Bailey, J.E. Measurements and models of synchronous growth of fission yeast induced by temperature oscillations. [Schizosaccharomyces pombe]. United States. doi:10.1002/bit.260240118.
Agar, D.W., and Bailey, J.E. Fri . "Measurements and models of synchronous growth of fission yeast induced by temperature oscillations. [Schizosaccharomyces pombe]". United States. doi:10.1002/bit.260240118.
@article{osti_5049033,
title = {Measurements and models of synchronous growth of fission yeast induced by temperature oscillations. [Schizosaccharomyces pombe]},
author = {Agar, D.W. and Bailey, J.E.},
abstractNote = {Pulsing of temperature in a fermentor at intervals coincident with cell generation time was used to induce synchrony in a population of the fission yeast Schizosaccharomyces pombe. Measurements of culture protein, RNA, and DNA during synchronous growth confirm continuous synthesis of protein and RNA and discontinuous synthesis of DNA as previously reported. Flow microfluorometry of populations at different times during the synchrony cycle was used to monitor the changes in single-cell protein, RNA, and DNA frequency functions. These measurements illustrate very clearly the degree of synchrony and patterns of macromolecular synthesis and also confirm previous estimates of the cellular protein contents characteristic of dividing cells. Additional insights into single-cell kinetics and division controls are provided by two-parameter flow microfluorometry measurements and by mathematical modeling of population dynamics. Such data are necessary foundations for robust population balance models of microbial processes. (Refs. 31).},
doi = {10.1002/bit.260240118},
journal = {Biotechnol. Bioeng.; (United States)},
number = ,
volume = 24:1,
place = {United States},
year = {Fri Jan 01 00:00:00 EST 1982},
month = {Fri Jan 01 00:00:00 EST 1982}
}