Specific depletion of the B-cell population induced by aberrant expression of human interferon regulatory factor 1 gene in transgenic mice

Journal Article · Tue Jan 15 00:00:00 EST 1991 · Proceedings of the National Academy of Sciences of the United States of America; (United States)

DOI:https://doi.org/10.1073/pnas.88.2.532· OSTI ID:5043695

Yamada, Gen; Miyamoto, Hajime; Nakano, Naoko; Itoh, Susumu; Taniguchi, Tadatsugu ^[1]; Ogawa, Minetaro; Akagi, Kiwamu; Miyazaki, Yunichi; Nishikawa, shinichi; Yamamura, Kenichi ^[2]

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1. Osaka Univ. (Japan)
2. Kumamoto Univ. Medical School (Japan)

Interferons (IFNs) are well known both as antiviral proteins and as potent regulators of cell growth and differentiation. In fact, IFNs inhibit growth of various normal and transformed cell types. Previously, a nuclear factor, IRF-1 (interferon regulatory factor 1), which binds to type I IFN and some IFN-inducible gene promoters, was identified and cloned. Since the IRF-1 gene is both virus and IFN inducible, an intriguing issue is raised as to whether the IRF-1 gene is functioning in IFN-mediated regulation of cell growth and differentiation. In this study, we generated transgenic mice carrying the human IRF-1 gene linked to the human immunoglobulin heavy-chain enhancer. In the transgenic mice, all the lymphoid tissues examined showed a dramatic reduction in the number of B lymphocytes (B cells). Preparation and analysis of bone marrow cells from the chimeric mice indicated that the bone marrow is the effective site for specific depletion of the B-cell population. In fact, transgenic bone marrow cells cocultured with a bone marrow-derived stromal cell line revealed an altered B-cell maturation pattern.

• OSTI ID: 5043695
• Journal Information: Proceedings of the National Academy of Sciences of the United States of America; (United States), Vol. 88:2; ISSN 0027-8424
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
GROWTH
INHIBITION
INTERFERON
GENE REGULATION
BONE MARROW CELLS
CHIMERAS
IMMUNOGLOBULINS
LYMPHOCYTES
TRANSCRIPTION
TRITIUM COMPOUNDS
TUMOR CELLS
ANIMAL CELLS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CONNECTIVE TISSUE CELLS
GLOBULINS
GROWTH FACTORS
HYDROGEN COMPOUNDS
LEUKOCYTES
LYMPHOKINES
MATERIALS
MITOGENS
MOSAICISM
ORGANIC COMPOUNDS
PROTEINS
SOMATIC CELLS
550201* - Biochemistry- Tracer Techniques