Human Fc. gamma. RIII: Cloning, expression, and identification of the chromosomal locus of two Fc receptors for IgG

Journal Article · Wed Feb 01 00:00:00 EST 1989 · Proceedings of the National Academy of Sciences of the United States of America; (USA)

DOI:https://doi.org/10.1073/pnas.86.3.1013· OSTI ID:5037246

Peltz, G A; Moore, K W ^[1]; Grundy, H O; Lebo, R V; Barsh, G S ^[2]; Yssel, H ^[3]

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1. DNAX Research Institute of Molecular and Cellular Biology, Palo Alto, CA (USA)
2. Univ. of California, San Francisco (USA)
3. UNICET Laboratory of Immunology, Dardilly (France)

A cDNA clone encoding a human receptor for the Fc portion of IgG Fc{gamma}RIII or CD16, was isolated from a human leukocyte library by a transient expression-immunoselection procedure. This cDNA (pGP5) encodes a 46-kDa phosphatidylinositol-linked cell surface protein with CD16 determinants and affinity for human IgG. The deduced protein sequence is most homologous to the murine receptor Fc{gamma}RII{alpha}, with slightly less homology to the human receptors Fc{gamma}RII and Fc{epsilon}RI. The cDNA hybridizes to a 2.2 kilobase mRNA in human leukocytes and a cloned human natural killer cell line. Fc{gamma}RIII is mapped to chromosome 1 by spot-blot analysis of sorted human chromosomes. Hybridization of Fc{gamma}RII and Fc{gamma}RIII probes to restriction digests of human genomic DNA separated by pulsed-field gel electrophoresis demonstrates physical linkage of the two genes within a maximum distance of 200 kilobases. The results identify a locus for at least two Fc{gamma}R genes on human chromosome 1.

• OSTI ID: 5037246
• Journal Information: Proceedings of the National Academy of Sciences of the United States of America; (USA), Vol. 86:3, Issue 3; ISSN 0027-8424
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
HETEROCHROMOSOMES
GENETIC MAPPING
IMMUNOGLOBULINS
RECEPTORS
RECOMBINANT DNA
DNA HYBRIDIZATION
DNA SEQUENCING
DNA-CLONING
ELECTROPHORESIS
LEUKOCYTES
PHOSPHORUS 32
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CHROMOSOMES
CLONING
DAYS LIVING RADIOISOTOPES
DNA
GLOBULINS
HYBRIDIZATION
ISOTOPES
LIGHT NUCLEI
MAPPING
MATERIALS
MEMBRANE PROTEINS
NUCLEI
NUCLEIC ACIDS
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
PHOSPHORUS ISOTOPES
PROTEINS
RADIOISOTOPES
STRUCTURAL CHEMICAL ANALYSIS
550201* - Biochemistry- Tracer Techniques