Recombination walking: Genetic selection of clones from pooled libraries of yeast artificial chromosomes by homologous recombination

Journal Article · Wed Sep 01 00:00:00 EDT 1993 · Proceedings of the National Academy of Sciences of the United States of America; (United States)

DOI:https://doi.org/10.1073/pnas.90.17.8118· OSTI ID:5031775

Miller, A M; Savinelli, E A; Couture, S M; Hannigan, G M; Han, Z; Selden, R F; Treco, D A ^[1]

---

1. Transkaryotic Therapies, Cambridge, MA (United States)

Recombination walking is based on the genetic selection of specific human clones from a yeast artificial chromosome (YAC) library by homologous recombination. The desired clone is selected from a pooled (unorderd) YAC library, eliminating labor-intensive steps typically used in organizing and maintaining ordered YAC libraries. Recombination walking represents an efficient approach to library screening and is well suited for chromosome-walking approaches to the isolation of genes associated with common diseases. 29 refs., 4 figs., 1 tab.

• OSTI ID: 5031775
• Journal Information: Proceedings of the National Academy of Sciences of the United States of America; (United States), Vol. 90:17; ISSN 0027-8424
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
DISEASES
GENETIC MAPPING
HUMAN CHROMOSOMES
GENE RECOMBINATION
YEASTS
CHROMOSOMES
EUMYCOTA
FUNGI
MAPPING
MICROORGANISMS
PLANTS
550400* - Genetics