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Title: Nonsaturable amino acid reabsorption in kidneys of normal and mercury-poisoned rabbits

Abstract

At high plasma concentrations, a high-capacity, low-affinity or nonsaturable flux (J{sub hc}) accounts for a residual fractional reabsorption of cycloleucine, aspartate, and AIB of approximately 50% of the filtered load in rabbits; J{sub hc} in micromoles per milliliter glomerular filtrate is reduced in Hg-poisoned animals. The nonsaturable flux of cycloleucine is characterized by a transepithelial transit time (TET) of approximately 2 min in control animals; it was consistently much longer in Hg-poisoned animals. The clearance ratio of creatinine/inulin averaged 1.0, and no J{sub hc} could be demonstrated for glucose. We conclude that J{sub hc} is a high-capacity, low-affinity amino acid flux which passes through an intracellular solute pool, and which is sensitive to Hg at both the brush border and the basolateral cell membrane. If calculation of the saturation constants of aspartate reabsorption is restricted to experiments in which U/P <1.0, i.e. where J{sub hc} is unlikely too contribute greatly to reabsorption, values some 20% lower than those previously reported are obtained; the Hg inhibition still is apparently uncompetitive in nature.

Authors:
;  [1]
  1. Univ. of Cincinnati, OH (USA)
Publication Date:
OSTI Identifier:
5030245
Resource Type:
Journal Article
Journal Name:
Proceedings of the Society for Experimental Biology and Medicine; (USA)
Additional Journal Information:
Journal Volume: 189:2; Journal ID: ISSN 0037-9727
Country of Publication:
United States
Language:
English
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; AMINO ACIDS; MEMBRANE TRANSPORT; KIDNEYS; SENSITIVITY; MERCURY; TOXICITY; RABBITS; ANIMALS; BODY; CARBOXYLIC ACIDS; ELEMENTS; MAMMALS; METALS; ORGANIC ACIDS; ORGANIC COMPOUNDS; ORGANS; VERTEBRATES; 560300* - Chemicals Metabolism & Toxicology

Citation Formats

Foulkes, E C, and Blanck, S. Nonsaturable amino acid reabsorption in kidneys of normal and mercury-poisoned rabbits. United States: N. p., 1988. Web. doi:10.3181/00379727-189-42802.
Foulkes, E C, & Blanck, S. Nonsaturable amino acid reabsorption in kidneys of normal and mercury-poisoned rabbits. United States. https://doi.org/10.3181/00379727-189-42802
Foulkes, E C, and Blanck, S. 1988. "Nonsaturable amino acid reabsorption in kidneys of normal and mercury-poisoned rabbits". United States. https://doi.org/10.3181/00379727-189-42802.
@article{osti_5030245,
title = {Nonsaturable amino acid reabsorption in kidneys of normal and mercury-poisoned rabbits},
author = {Foulkes, E C and Blanck, S},
abstractNote = {At high plasma concentrations, a high-capacity, low-affinity or nonsaturable flux (J{sub hc}) accounts for a residual fractional reabsorption of cycloleucine, aspartate, and AIB of approximately 50% of the filtered load in rabbits; J{sub hc} in micromoles per milliliter glomerular filtrate is reduced in Hg-poisoned animals. The nonsaturable flux of cycloleucine is characterized by a transepithelial transit time (TET) of approximately 2 min in control animals; it was consistently much longer in Hg-poisoned animals. The clearance ratio of creatinine/inulin averaged 1.0, and no J{sub hc} could be demonstrated for glucose. We conclude that J{sub hc} is a high-capacity, low-affinity amino acid flux which passes through an intracellular solute pool, and which is sensitive to Hg at both the brush border and the basolateral cell membrane. If calculation of the saturation constants of aspartate reabsorption is restricted to experiments in which U/P <1.0, i.e. where J{sub hc} is unlikely too contribute greatly to reabsorption, values some 20% lower than those previously reported are obtained; the Hg inhibition still is apparently uncompetitive in nature.},
doi = {10.3181/00379727-189-42802},
url = {https://www.osti.gov/biblio/5030245}, journal = {Proceedings of the Society for Experimental Biology and Medicine; (USA)},
issn = {0037-9727},
number = ,
volume = 189:2,
place = {United States},
year = {Tue Nov 01 00:00:00 EST 1988},
month = {Tue Nov 01 00:00:00 EST 1988}
}