Enhanced poly(adenosine diphosphate ribose) polymerase activity and gene expression in Ewing's sarcoma cells

Journal Article · Mon Jan 01 00:00:00 EST 1990 · Cancer Research; (USA)

OSTI ID:5030009

Prasad, S C; Thraves, P J; Bhatia, K G; Smulson, M E; Dritschilo, A ^[1]

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1. Georgetown Univ. Medical Center, Washington, DC (USA)

Ewing's sarcoma (ES) is a highly malignant childhood bone tumor and is considered curable by moderate doses of radiotherapy. The addition of chemical inhibitors of the activity of the nuclear enzyme poly(adenosine diphosphate ribose) (poly(ADPR)) polymerase to ES cells in culture results in increased cell killing, a phenomenon called inhibitor sensitization. Since poly(ADPR) polymerase is thought to be associated with DNA repair, it has been suggested that ES cells and other inhibitor-sensitized cells may have a reduced capacity for polymer synthesis resulting in deficient postirradiation recovery. We present here the unexpected observation that in comparison to other cell lines tested, ES cells exhibit a high enzyme activity, higher constitutive levels of the protein, and elevated levels of its mRNA transcript for poly(ADPR) polymerase. No gross amplifications or rearrangements of the gene were observed; however, regulation of poly(ADPR) polymerase in these tumor cells takes place at the level of the gene transcript.

• OSTI ID: 5030009
• Journal Information: Cancer Research; (USA), Vol. 50:1; ISSN 0008-5472
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
POLYMERASES
ENZYME ACTIVITY
TUMOR CELLS
DNA REPAIR
DNA HYBRIDIZATION
GENE REGULATION
HELA CELLS
MESSENGER-RNA
SARCOMAS
TRANSCRIPTION
ANIMAL CELLS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
DISEASES
ENZYMES
HYBRIDIZATION
NEOPLASMS
NUCLEIC ACIDS
NUCLEOTIDYLTRANSFERASES
ORGANIC COMPOUNDS
PHOSPHORUS-GROUP TRANSFERASES
RECOVERY
REPAIR
RNA
TRANSFERASES
550201* - Biochemistry- Tracer Techniques
550901 - Pathology- Tracer Techniques